Injection of antisense oligos to nNOS into nucleus tractus solitarii increases blood pressure. 1999

M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
Department of Systems Physiology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan.

We investigated the cardiovascular effects of bilateral microinjection of antisense oligodeoxynucleotides (oligos) into the nucleus tractus solitarii (NTS) to neuronal nitric oxide synthase (nNOS) to suppress the expression of nNOS molecular biologically. In urethane-anesthetized, paralyzed Wistar-Kyoto rats, bilateral microinjection of nNOS antisense oligos (20 pmol in a 50nl volume) into the NTS produced a significant increase in mean arterial blood pressure at 30-60min after injection, compared with rats injected with nNOS sense or scrambled oligos. Immunohistochemical study demonstrated that nNOS immunoreactivity in the rat NTS was suppressed by nNOS antisense oligos. These results indicate that suppression of the nNOS gene using antisense in the NTS increases blood pressure.

UI MeSH Term Description Entries
D008297 Male Males
D008845 Microinjections The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes. Microinjection
D009569 Nitric Oxide A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP. Endogenous Nitrate Vasodilator,Mononitrogen Monoxide,Nitric Oxide, Endothelium-Derived,Nitrogen Monoxide,Endothelium-Derived Nitric Oxide,Monoxide, Mononitrogen,Monoxide, Nitrogen,Nitrate Vasodilator, Endogenous,Nitric Oxide, Endothelium Derived,Oxide, Nitric,Vasodilator, Endogenous Nitrate
D011921 Rats, Inbred WKY A strain of Rattus norvegicus used as a normotensive control for the spontaneous hypertensive rats (SHR). Rats, Wistar Kyoto,Wistar Kyoto Rat,Rats, WKY,Inbred WKY Rat,Inbred WKY Rats,Kyoto Rat, Wistar,Rat, Inbred WKY,Rat, WKY,Rat, Wistar Kyoto,WKY Rat,WKY Rat, Inbred,WKY Rats,WKY Rats, Inbred,Wistar Kyoto Rats
D001784 Blood Gas Analysis Measurement of oxygen and carbon dioxide in the blood. Analysis, Blood Gas,Analyses, Blood Gas,Blood Gas Analyses,Gas Analyses, Blood,Gas Analysis, Blood
D001794 Blood Pressure PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS. Systolic Pressure,Diastolic Pressure,Pulse Pressure,Pressure, Blood,Pressure, Diastolic,Pressure, Pulse,Pressure, Systolic,Pressures, Systolic
D006339 Heart Rate The number of times the HEART VENTRICLES contract per unit of time, usually per minute. Cardiac Rate,Chronotropism, Cardiac,Heart Rate Control,Heartbeat,Pulse Rate,Cardiac Chronotropy,Cardiac Chronotropism,Cardiac Rates,Chronotropy, Cardiac,Control, Heart Rate,Heart Rates,Heartbeats,Pulse Rates,Rate Control, Heart,Rate, Cardiac,Rate, Heart,Rate, Pulse
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015971 Gene Expression Regulation, Enzymologic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis. Enzymologic Gene Expression Regulation,Regulation of Gene Expression, Enzymologic,Regulation, Gene Expression, Enzymologic
D016375 Antisense Elements (Genetics) Nucleic acids which hybridize to complementary sequences in other target nucleic acids causing the function of the latter to be affected. Antisense Probes,Anti-Sense Elements,Anti-Sense Probes,Anti Sense Elements,Anti Sense Probes,Elements, Anti-Sense,Probes, Anti-Sense,Probes, Antisense

Related Publications

M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
January 1990, Clinical and experimental hypertension. Part A, Theory and practice,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
November 2000, Pharmacology, biochemistry, and behavior,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
November 2008, Autonomic neuroscience : basic & clinical,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
March 1992, Brain research,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
July 1985, European journal of pharmacology,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
January 1981, European journal of pharmacology,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
November 2019, Acta Cardiologica Sinica,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
August 2023, Neuroscience bulletin,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
July 2022, Journal of neurophysiology,
M Maeda, and H Hirano, and H Kudo, and Y Doi, and K Higashi, and S Fujimoto
January 2013, Circulation journal : official journal of the Japanese Circulation Society,
Copied contents to your clipboard!