Biomaterial Database

Direct determination of tramadol glucuronides in human urine by high-performance liquid chromatography with fluorescence detection. 1999

P Overbeck, and G Blaschke

Institute of Pharmaceutical Chemistry, University of Münster, Germany.

A sensitive and selective reversed-phase high-performance liquid chromatography method has been developed for the direct determination of three glucuronides of the centrally acting analgesic tramadol (1). Separation of these glucuronides into their diastereomers was achieved by HPLC using ion pair chromatography with nonanesulfonic acid sodium salt and LiChrospher 100 RP 18 as stationary phase. Quantification of O-demethyltramadol glucuronide and N,O-didemethyltramadol glucuronide in human urine was performed by fluorescence detection. The urine samples were purified by a two-step solid-phase extraction. The glucuronides were found to be highly enriched in the 1S,2S-diastereomers. The results of a study with three healthy volunteers are presented.

UI	MeSH Term	Description	Entries
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000284	Administration, Oral	The giving of drugs, chemicals, or other substances by mouth.	Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations
D000701	Analgesics, Opioid	Compounds with activity like OPIATE ALKALOIDS, acting at OPIOID RECEPTORS. Properties include induction of ANALGESIA or NARCOSIS.	Opioid,Opioid Analgesic,Opioid Analgesics,Opioids,Full Opioid Agonists,Opioid Full Agonists,Opioid Mixed Agonist-Antagonists,Opioid Partial Agonists,Partial Opioid Agonists,Agonist-Antagonists, Opioid Mixed,Agonists, Full Opioid,Agonists, Opioid Full,Agonists, Opioid Partial,Agonists, Partial Opioid,Analgesic, Opioid,Full Agonists, Opioid,Mixed Agonist-Antagonists, Opioid,Opioid Agonists, Full,Opioid Agonists, Partial,Opioid Mixed Agonist Antagonists,Partial Agonists, Opioid
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013237	Stereoisomerism	The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)	Molecular Stereochemistry,Stereoisomers,Stereochemistry, Molecular,Stereoisomer
D014147	Tramadol	A narcotic analgesic proposed for severe pain. It may be habituating.	Adolonta,Amadol,Biodalgic,Biokanol,Contramal,Jutadol,K-315,MTW-Tramadol,Nobligan,Prontofort,Ranitidin 1A Pharma,Takadol,Theradol,Tiral,Topalgic,Tradol,Tradol-Puren,Tradonal,Tralgiol,Trama 1A Pharma,Trama AbZ,Trama KD,Trama-Dorsch,Tramabeta,Tramadin,Tramadoc,Tramadol 1A,Tramadol AL,Tramadol Asta Medica,Tramadol Basics,Tramadol Bayvit,Tramadol Bexal,Tramadol Cinfa,Tramadol Edigen,Tramadol Heumann,Tramadol Hydrochloride,Tramadol Kern,Tramadol Lichtenstein,Tramadol Lindo,Tramadol Mabo,Tramadol Normon,Tramadol PB,Tramadol Ratiopharm,Tramadol Stada,Tramadol acis,Tramadol-Dolgit,Tramadol-Hameln,Tramadol-ratiopharm,Tramadolor,Tramadura,Tramagetic,Tramagit,Tramake,Tramal,Tramex,Tramundin,Trasedal,Ultram,Xymel 50,Zamudol,Zumalgic,Zydol,Zytram,K 315,K315,MTW Tramadol,MTWTramadol,Tradol Puren,TradolPuren,Trama Dorsch,TramaDorsch,Tramadol Dolgit,Tramadol Hameln,TramadolDolgit,TramadolHameln,Tramadolratiopharm
D020719	Glucuronides	Glycosides of GLUCURONIC ACID formed by the reaction of URIDINE DIPHOSPHATE GLUCURONIC ACID with certain endogenous and exogenous substances. Their formation is important for the detoxification of drugs, steroid excretion and BILIRUBIN metabolism to a more water-soluble compound that can be eliminated in the URINE and BILE.	Glucuronide