BIOMAT Database Logo BIOMAT Database Logo

Tissue-non-specific alkaline phosphatase mRNA expression and alkaline phosphatase activity following application of retinoic acid in cultured human dental pulp cells. 1999

S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan. symone.peri@dent.tmd.ac.jp

Retinoic acid is a potent inducer of tissue-non-specific alkaline phosphatase (TNSALP) expression in various osteoblastic and fibroblastic cells, and may be involved in morphogenesis, cellular growth and differentiation. This study investigates the effects of retinoic acid on alkaline phosphatase activity and TNSALP gene expression in human dental pulp cells. Cultured cells were treated with various concentrations of retinoic acid (0, 10(-7), 10(- 6), 10 (-5) M) in 0.5% bovine serum albumin without serum. Alkaline phosphatase activity was determined by the rate of p-nitrophenyl phosphate hydrolysis and was also assayed in the presence of various inhibitors and under thermal inactivation. A set of specific oligonucleotide primers was selected, based on the nucleotide sequences of two human TNSALP mRNA (bone and liver) types, and reverse transcription-polymerase chain reaction (RT-PCR) performed. Inhibitory and thermal inactivation experiments revealed that the elevated alkaline phosphatase activity had properties of the TNSALP type. RT-PCR showed that retinoic acid enhanced the expression of bone-type TNSALP mRNA in pulp cells. However, the liver-type TNSALP mRNA was not detected. These findings suggest that the high alkaline phosphatase activity of retinoic acid-treated dental pulp cells is associated with increased transcription of the bone-type mRNA of the TNSALP gene and not with liver-type.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003782 Dental Pulp A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992) Dental Pulps,Pulp, Dental,Pulps, Dental
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004790 Enzyme Induction An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis. Induction, Enzyme
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000469 Alkaline Phosphatase An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
D000704 Analysis of Variance A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable. ANOVA,Analysis, Variance,Variance Analysis,Analyses, Variance,Variance Analyses
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Vitamin D and tissue non-specific alkaline phosphatase in dental cells.
May 2006, European journal of oral sciences,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Expression of mRNA encoding tissue-nonspecific alkaline phosphatase in human dental tissues.
February 1999, Calcified tissue international,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Tissue non-specific alkaline phosphatase production by human dental pulp stromal cells is enhanced by high density cell culture.
August 2015, Cell and tissue research,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Bovine enamel organ cells express tissue non-specific alkaline phosphatase mRNA.
December 1995, Journal of dental research,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Retinoic acid and methylation cis-regulatory elements control the mouse tissue non-specific alkaline phosphatase gene expression.
June 1996, Mechanisms of development,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Layer-specific activity of tissue non-specific alkaline phosphatase in the human neocortex.
January 2011, Neuroscience,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
[Distribution of alkaline phosphatase in human dental pulp].
August 1950, Revista odontologica,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
[Phosphatases in dental pulp. I. Acid and alkaline phosphatase].
November 1965, Czasopismo stomatologiczne,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
Heat stress induces alkaline phosphatase activity and heat shock protein 25 expression in cultured pulp cells.
February 2008, International endodontic journal,
S M San Miguel, and M Goseki-Sone, and E Sugiyama, and H Watanabe, and M Yanagishita, and I Ishikawa
[Alkaline phosphatase activity in human dental pulp under normal and pathological conditions].
December 1979, Fogorvosi szemle,
Copied contents to your clipboard!