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Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid recombinase gene. We increased the production and secretion of two heterologous proteins, glucoamylase from the yeast Arxula adeninivorans and mammalian interleukin-1beta, following gene dosage amplification when the heterologous genes were carried by pKD1-based vectors. The choice of the promoters for expression of the integrated recombinase gene and of the episomal heterologous genes are critical for the mitotic stability of the host-vector system.
G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
May 2006,
FEMS yeast research,
G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
August 2019,
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G B Morlino, and
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L Frontali, and
M M Bianchi
June 1996,
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G B Morlino, and
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M M Bianchi
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Antonie van Leeuwenhoek,
G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
April 2004,
Journal of biotechnology,
G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
December 2008,
Applied and environmental microbiology,
G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
February 2002,
Applied microbiology and biotechnology,
G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
May 1998,
Yeast (Chichester, England),
G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
January 2012,
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G B Morlino, and
L Tizzani, and
R Fleer, and
L Frontali, and
M M Bianchi
May 2009,
Biotechnology and applied biochemistry,
