BIOMAT Database Logo BIOMAT Database Logo

Effect of dental materials on gluconeogenesis in rat kidney tubules. 1999

F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Walther-Straub-Institute of Pharmacology and Toxicology, Ludwig-Maximilians-University of Munich, Nussbaumstrasse 26, D-80336 Munich, Germany. F.X.Reichl@lrz.uni-muenchen.de

The effect of dental composite components triethyleneglycoldimethacrylate (TEGDMA) and hydroxyethylmethacrylate (HEMA) as well as mercuric chloride (HgCl(2)) and methylmercury chloride (MeHgCl) on gluconeogenesis was investigated in isolated rat kidney tubules. From starved rats kidney tubules were prepared and isolated by digestion with collagenase. Every 10 min up to 60 min 1-ml samples were drawn from the cell suspension for quantitating the glucose content. Glucose formation in controls was 3.3 +/- 0.2 nmol/mg. per min (mean +/- SEM, n=21). Relative rates of glucose formation were obtained by expressing individual rates as a percentage of the corresponding control. X-Y concentration curves (effective concentration, EC) of the substances were calculated by fitting a four-parametric sigmoid function to the relative rates of glucose formation at various test concentrations. At the end of the incubation period cell viability was assessed by trypan blue exclusion. Cell viability decreased within the 60 min interval from 90 to approx. 80% (controls), <25 (HEMA), <20 (TEGDMA), <10 (MeHgCl), and <10% (HgCl(2)). Values of 50% effective concentration (EC(50)) were calculated from fitted curves. EC(50) values were (mmol; mean +/- SEM; n=4): HEMA, 17.7 +/- 2.9; TEGDMA, 1.8 +/- 0.2; MeHgCl, 0.018 +/- 0.0005; and HgCl(2), 0. 0016 +/- 0.0005. The toxic effect of HgCl(2) was approximately 1000 or 10 000 higher than that of the dental composite components TEGDMA or HEMA, respectively.

UI MeSH Term Description Entries
D007684 Kidney Tubules Long convoluted tubules in the nephrons. They collect filtrate from blood passing through the KIDNEY GLOMERULUS and process this filtrate into URINE. Each renal tubule consists of a BOWMAN CAPSULE; PROXIMAL KIDNEY TUBULE; LOOP OF HENLE; DISTAL KIDNEY TUBULE; and KIDNEY COLLECTING DUCT leading to the central cavity of the kidney (KIDNEY PELVIS) that connects to the URETER. Kidney Tubule,Tubule, Kidney,Tubules, Kidney
D008297 Male Males
D008627 Mercuric Chloride Mercury chloride (HgCl2). A highly toxic compound that volatizes slightly at ordinary temperature and appreciably at 100 degrees C. It is corrosive to mucous membranes and used as a topical antiseptic and disinfectant. Mercury Dichloride,Corrosive Sublimate,HgCl2,Mercuric Perchloride,Mercury Bichloride,Mercury Perchloride,Sublimate,Bichloride, Mercury,Chloride, Mercuric,Dichloride, Mercury,Perchloride, Mercuric,Perchloride, Mercury,Sublimate, Corrosive
D008689 Methacrylates Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group. Methacrylate
D008767 Methylmercury Compounds Organic compounds in which mercury is attached to a methyl group. Methyl Mercury Compounds,Compounds, Methyl Mercury,Compounds, Methylmercury,Mercury Compounds, Methyl
D011092 Polyethylene Glycols Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS. Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D011109 Polymethacrylic Acids Poly-2-methylpropenoic acids. Used in the manufacture of methacrylate resins and plastics in the form of pellets and granules, as absorbent for biological materials and as filters; also as biological membranes and as hydrogens. Synonyms: methylacrylate polymer; poly(methylacrylate); acrylic acid methyl ester polymer. Methacrylic Acid Polymers,Acid Polymers, Methacrylic,Acids, Polymethacrylic,Polymers, Methacrylic Acid
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D003188 Composite Resins Synthetic resins, containing an inert filler, that are widely used in dentistry. Composite Resin,Resin, Composite,Resins, Composite
D005943 Gluconeogenesis Biosynthesis of GLUCOSE from nonhexose or non-carbohydrate precursors, such as LACTATE; PYRUVATE; ALANINE; and GLYCEROL.

Related Publications

F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Synergistic effects of H2O2 with components of dental restorative materials on gluconeogenesis in rat kidney tubules.
May 2003, Biomaterials,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Effect of alpha-adrenergic agonists on gluconeogenesis and 45Ca efflux in rat kidney tubules.
July 1982, Biochemical pharmacology,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Effect of glycerol on gluconeogenesis in isolated rabbit kidney cortex tubules.
July 1988, Biochimica et biophysica acta,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Effect of starvation on glutamine ammoniagenesis and gluconeogenesis in isolated mouse kidney tubules.
November 2002, The Biochemical journal,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
On the stimulation of gluconeogenesis by L-lysine in isolated rat kidney cortex tubules.
June 1975, Biochimica et biophysica acta,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Inhibition of gluconeogenesis in isolated rat kidney tubules by branched chain alpha-ketoacids.
November 1978, Pediatric research,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Effect of compound D-600 (methoxyverapamil) on gluconeogenesis and on acceleration of the process by alpha-adrenergic stimuli in rat kidney tubules.
August 1980, The Biochemical journal,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Effect of adrenalectomy on acceleration of gluconeogenesis by calcium ions, adenosine 3':5'-cyclic monophosphate and adrenaline in rat kidney tubules.
August 1978, The Biochemical journal,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Gluconeogenesis in renal cortical tubules. Effect of phenformin.
January 1973, Diabetes,
F X Reichl, and J Durner, and H Mückter, and B Elsenhans, and W Forth, and K H Kunzelmann, and R Hickel, and W Spahl, and W R Hume, and G W Moes
Metabolism of isolated kidney tubules. Oxygen consumption, gluconeogenesis and the effect of cyclic nucleotides in tubules from starved rats.
October 1971, Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
Copied contents to your clipboard!