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Purified Newcastle disease virus contains an enzyme that incorporates the methyl group from S-adenosyl-L-methionine into RNA synthesized in vitro by the virion-associated RNA polymerase (RNA nucleotidyltransferase). Incorporation of radioactivity from S-adenosyl-L-[methyl-3H]methionine was totally dependent upon RNA synthesis. The methylation reaction was completely inhibited by S-adenosyl-L-homocysteine, suggesting the transfer of only the methyl group of S-adenosyl-methionine to RNA products. Velocity sedimentation and hybridization of the in vitro product RNA indicated that both [3H]methyl and [32P]GMP labels resided in single-stranded 18S RNA molecules which were virus specific. Approximately 1 to 2 methyl groups were incorporated per RNA molecule. DEAE-cellulose chromatography of product RNA after alkaline hydrolysis suggested that the 5' terminus was the site of methylation.
R J Colonno, and
H O Stone
January 1975,
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January 1970,
Doklady Akademii nauk SSSR,
R J Colonno, and
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Cell,
R J Colonno, and
H O Stone
August 1977,
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R J Colonno, and
H O Stone
January 1972,
Voprosy virusologii,
R J Colonno, and
H O Stone
May 1982,
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R J Colonno, and
H O Stone
August 1974,
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R J Colonno, and
H O Stone
July 1977,
Virology,
R J Colonno, and
H O Stone
January 1976,
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R J Colonno, and
H O Stone
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Biochemical and biophysical research communications,
