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OBJECTIVE We previously reported a novel cytoskeletal protein with a myosin-like domain which is localized in the ciliary rootlet and basal body of connecting cilium of photoreceptor and hence we named it 'myocilin'. It was soon realized that myocilin is identical to a protein called TIGR (trabecular meshwork inducible glucocorticoid response protein) which was found to be responsible for the pathogenesis of juvenile open angle glaucoma. In this study, we employed in situ RNA hybridization to examine the myocilin (MYOC)/ TIGR gene expression in the trabecular meshworks of glaucomatous and nonglaucomatous eyes. METHODS The glaucomatous specimens were obtained by trabeculectomy from the patients with primary open angle glaucoma (POAG), chronic angle closure glaucoma (CACG) and steroid glaucoma, respectively, and the nonglaucomatous specimens were obtained from a victim of traffic accident at autopsy and from a patient with maxillary sinus carcinoma at enucleation for the operation. The in situ RNA hybridization was carried out with digoxigenin-labeled sense and antisense RNA probes. RESULTS In all cases, hybridization signals were detected primarily in the trabecular meshwork cells and secondarily in the fibroblast-like cells of corneoscleral wall. CONCLUSIONS Myocilin gene is expressed clearly in the trabecular meshwork cells of both glaucomatous and nonglaucomatous eyes.
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
June 2000,
Eye (London, England),
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
October 1999,
Investigative ophthalmology & visual science,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
July 2002,
International journal of molecular medicine,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
May 2011,
Vision research,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
July 2004,
Experimental cell research,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
August 2005,
The Journal of biological chemistry,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
February 2003,
Genetics,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
June 2006,
Yan ke xue bao = Eye science,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
July 2004,
Journal of cellular physiology,
H Takahashi, and
S Noda, and
Y Mashima, and
R Kubota, and
Y Ohtake, and
T Tanino, and
J Kudoh, and
S Minoshima, and
Y Oguchi, and
N Shimizu
June 2006,
Experimental eye research,
