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Synaptobrevin-2, syntaxin-1, and SNAP-25 were identified in rat alveolar epithelial type II cells by Western blot analysis. Synaptobrevin-2 was localized in the lamellar bodies, and syntaxin-1 and SNAP-25 were found in 0.4% Nonidet P40-soluble and -insoluble fractions, respectively, of the type II cells. When the isolated type II cells were stimulated for secretion with calcium ionophore A23187 or with phorbol 12-myristate 13-acetate, these proteins were found to have been proteolyzed. Preincubation of cells with calpain inhibitor II (N-acetylleucylleucylmethionine), however, prevented the proteolysis. Treatment of the cell lysate with exogenous calpain resulted in a time-dependent decrease of these proteins. The data suggest that synaptobrevin, syntaxin, and SNAP-25 are subject to proteolytic modification by activated calpain in intact type II cells stimulated for secretion.
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
December 1995,
FEBS letters,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
December 1996,
Neurochemistry international,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
May 1997,
Journal of neuroscience research,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
May 2013,
Cell reports,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
September 1996,
FEBS letters,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
February 2008,
Structure (London, England : 1993),
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
June 1998,
Neuroreport,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
February 2010,
The Journal of cell biology,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
October 1992,
Biochimica et biophysica acta,
U J Zimmerman, and
S K Malek, and
L Liu, and
H L Li
June 1997,
Proceedings of the National Academy of Sciences of the United States of America,
