Biomaterial Database

[Cruzipain, major cysteine proteinase of Trypanosoma cruzi: sequence and genomic organization of the codifying genes]. 1999

J J Cazzulo

Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Provincia de Buenos Aires, Argentina. jcazzulo@iib.unsam.edu.ar

Cruzipain is the major cysteine proteinase present in Trypanosoma cruzi, the parasite causing the American Trypanosomiasis, Chagas disease. The enzyme is encoded by a high number of genes (up to 130 in the Tul 2 stock) placed in head-to-tail tandems, and located in two to four chromosomes. The simultaneous expression of several different genes results in the production of a complex mixture of isoforms. Those known as a group as "cruzipain 1" differ essentially at the level of the C-terminal domain, in their amino-acid sequence and in their type of N-glycosylation. The existence of a more distantly related cysteine proteinase, "cruzipain 2", has been demonstrated; it differs markedly, particularly at the level of the catalytic moiety.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003546	Cysteine Endopeptidases	ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014349	Trypanosoma cruzi	The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.	Trypanosoma cruzus,cruzi, Trypanosoma
D015800	Protozoan Proteins	Proteins found in any species of protozoan.	Proteins, Protozoan
D018503	Genome, Protozoan	The complete genetic complement contained in a set of CHROMOSOMES in a protozoan.	Protozoan Genome,Genomes, Protozoan,Protozoan Genomes