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A rapid but sensitive radioimmunoassay for the measurement of plasma angiotensin II has been developed. Antiserum to angiotensin II coupled to albumin was raised and boosted by subsequent injections of unconjugated hormone. The antibody was found to react equally with the Val5 and Ileu5 forms of angiotensin II as well as with the hexa and heptapeptide fragments. Only minor cross reactivity with angiotensin I was observed. The minimum amount of angiotensin II that could be reliably measured was 4pg per tube. Because plasma incubates resulted in large and variable losses in sensitivity, an ethanol precipitation step was employed for plasma samples prior to radioimmunoassay. The normal range of venous angiotensin II levels in ambulant volunteers was 20-83pg/ml. Highly significant correlations of plasma angiotensin II with simultaneously drawn samples for renin determination were noted. Venous angiotensin II levels in a number of clinical situations are documented.
M G Nicholls, and
E A Espiner
January 1973,
Acta endocrinologica. Supplementum,
M G Nicholls, and
E A Espiner
October 1994,
Journal of pharmacological and toxicological methods,
M G Nicholls, and
E A Espiner
August 1979,
Japanese circulation journal,
M G Nicholls, and
E A Espiner
June 1983,
Steroids,
M G Nicholls, and
E A Espiner
December 1985,
Endocrinologia japonica,
M G Nicholls, and
E A Espiner
November 1977,
Clinica chimica acta; international journal of clinical chemistry,
M G Nicholls, and
E A Espiner
January 1975,
Antimicrobial agents and chemotherapy,
M G Nicholls, and
E A Espiner
January 1983,
Vision research,
M G Nicholls, and
E A Espiner
July 1969,
Proceedings of the Royal Society of London. Series B, Biological sciences,
M G Nicholls, and
E A Espiner
March 1974,
Biomedicine / [publiee pour l'A.A.I.C.I.G.],
