Biomaterial Database

Ultrastructural effects of chemical agents and moist heat on Bacillus subtilis. I--Effects on vegetative cells. 1978

M T Silva, and J C Sousa, and G Balassa

The ultrastructural alterations induced by treatment of vegetative Bacillus subtilis cells with organic solvents, trichloroacetic acid (TCA) and moist heat were examined by electron microscopy. Organic solvents disorganize the membrane and change the asymmetric unit membrane profile to a symmetric profile. They also lead to partial solubilization of the membrane and produce small or extensive gaps. Membrane damaging activity increases in the following order: xylene = toluene less than octanol less than choroform. TCA coagulates the cytoplasm which shows large, electron-dense, pronase-sensitive blocks. Moist heat alters both the membrane and the cytoplasm.

UI	MeSH Term	Description	Entries
D007425	Intracellular Membranes	Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.	Membranes, Intracellular,Intracellular Membrane,Membrane, Intracellular
D002725	Chloroform	A commonly used laboratory solvent. It was previously used as an anesthetic, but was banned from use in the U.S. due to its suspected carcinogenicity.	Trichloromethane
D003593	Cytoplasm	The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)	Protoplasm,Cytoplasms,Protoplasms
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000442	Octanols	Isomeric forms and derivatives of octanol (C8H17OH).	Alcohols, Octyl,Heptylcarbinols,Hydroxyoctanes,Octylic Alcohols,Alcohols, Octylic,Octyl Alcohols
D001412	Bacillus subtilis	A species of gram-positive bacteria that is a common soil and water saprophyte.	Natto Bacteria,Bacillus subtilis (natto),Bacillus subtilis subsp. natto,Bacillus subtilis var. natto
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D012997	Solvents	Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)	Solvent
D014050	Toluene	A widely used industrial solvent.
D014238	Trichloroacetic Acid	A strong acid used as a protein precipitant in clinical chemistry and also as a caustic for removing warts.	Acide trichloracetique,Rubidium Trichloroacetate,Sodium Trichloroacetate,Acid, Trichloroacetic,Trichloroacetate, Rubidium,Trichloroacetate, Sodium,trichloracetique, Acide