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We made an artificial skin comprised of a stratified layer of keratinocytes and a dermal matrix with a type I collagen containing fibroblasts. In this work, we showed keratinocyte behavior under primary culture, gel contractions varying with concentration of collagen solution, and cell growth plots in the collagen gel. The optimum behavior of dermal equivalent could be obtained using 3.0 mg/ml collagen solution and attached gel culture. The attached gel culture had a jumping effect of growth factor on cell growth at the lag phase. To develop the artificial skin, 1x10(5) cells/cm2 of keratinocytes were cultured on the dermal equivalent at air-liquid interface. Finally, to overcome the problem that artificial skin of collagen gel was torn easily during suturing of grafting, we prepared histocompatible collagen mesh and attached the mesh to the bottom of the gel. Cultured artificial skins were successfully grafted onto rats.
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
March 2006,
Sao Paulo medical journal = Revista paulista de medicina,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
November 2020,
Plastic and reconstructive surgery,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
November 2020,
Plastic and reconstructive surgery,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
January 1998,
Journal of hand therapy : official journal of the American Society of Hand Therapists,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
September 1970,
The Journal of investigative dermatology,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
January 2004,
Advances in gerontology = Uspekhi gerontologii,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
May 2018,
Journal of microscopy,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
April 2019,
Advanced healthcare materials,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
January 2013,
Journal of biomaterials science. Polymer edition,
E K Yang, and
Y K Seo, and
H H Youn, and
D H Lee, and
S N Park, and
J K Park
September 2012,
Pharmaceutical research,
