BIOMAT Database Logo BIOMAT Database Logo

De novo RNA synthesis catalyzed by HCV RNA-dependent RNA polymerase. 2000

X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
Infectious Diseases Research, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.

The 65 kDa RNA-dependent RNA polymerase (NS5B), encoded by the hepatitis C virus (HCV) genome, is a key component involved in viral replication. Here we provide the direct evidence that purified HCV polymerase catalyzed de novo RNA synthesis in a primer-independent manner using homopolymers and HCV RNA as templates. The enzyme could utilize both polyC and polyU as templates for de novo RNA synthesis, suggesting that NS5B specifically recognized pyrimidine bases for initiation. More importantly, NS5B also catalyzed de novo RNA synthesis with an HCV RNA template; the resulting nascent RNA products, smaller than the template used, contained ATP as the first nucleotide. These results indicate that the newly synthesized RNAs did not result from template self-priming and suggest that a replication initiation site in the HCV RNA genome is a uridylate.

UI MeSH Term Description Entries
D011066 Poly C A group of cytosine ribonucleotides in which the phosphate residues of each cytosine ribonucleotide act as bridges in forming diester linkages between the ribose moieties. Cytosine Polynucleotides,Polycytidylic Acid,Polycytidylic Acids,Acid, Polycytidylic,Acids, Polycytidylic,Polynucleotides, Cytosine
D011072 Poly U A group of uridine ribonucleotides in which the phosphate residues of each uridine ribonucleotide act as bridges in forming diester linkages between the ribose moieties. Polyuridylic Acids,Uracil Polynucleotides,Poly(rU),Acids, Polyuridylic,Polynucleotides, Uracil
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D012324 RNA-Dependent RNA Polymerase An enzyme that catalyses RNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time, and can initiate a chain de novo. (Enzyme Nomenclature, 1992, p293) Nucleoside-Triphosphate:RNA Nucleotidyltransferase (RNA-directed),RNA Replicase,RNA-Dependent RNA Replicase,RNA-Directed RNA Polymerase,RNA Dependent RNA Polymerase,RNA Dependent RNA Replicase,RNA Directed RNA Polymerase,RNA Polymerase, RNA-Dependent,RNA Polymerase, RNA-Directed,RNA Replicase, RNA-Dependent,Replicase, RNA,Replicase, RNA-Dependent RNA
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D016174 Hepacivirus A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species. Hepatitis C virus,Hepatitis C-Like Viruses,Hepaciviruses,Hepatitis C Like Viruses,Hepatitis C viruses,Hepatitis C-Like Virus
D016679 Genome, Viral The complete genetic complement contained in a DNA or RNA molecule in a virus. Viral Genome,Genomes, Viral,Viral Genomes
D017361 Viral Nonstructural Proteins Proteins encoded by a VIRAL GENOME that are not structural components of VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY. Nonstructural Proteins, Viral,NS Proteins, Viral,Viral NS Proteins,Viral Non-Structural Proteins,Viral Nonstructural Protein,Viral Nonstructural Proteins NS1,Viral Nonstructural Proteins NS2,Nonstructural Protein, Viral,Viral Non Structural Proteins
D020413 3' Untranslated Regions The sequence at the 3' end of messenger RNA that does not code for product. This region contains transcription and translation regulating sequences. 3'UTR,3' UTR,3' Untranslated Region,3' UTRs,3'UTRs,Region, 3' Untranslated,Regions, 3' Untranslated,UTR, 3',UTRs, 3',Untranslated Region, 3',Untranslated Regions, 3'

Related Publications

X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
De novo RNA synthesis catalyzed by the Zika Virus RNA polymerase domain.
June 2017, Scientific reports,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
De novo initiation of RNA synthesis by the arterivirus RNA-dependent RNA polymerase.
August 2007, Journal of virology,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase.
April 2016, Molecular and cellular biology,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
De novo initiation of RNA synthesis by a recombinant flaviviridae RNA-dependent RNA polymerase.
January 1999, Virology,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase.
December 2003, European journal of biochemistry,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
6S RNA-dependent inhibition of RNA polymerase is released by RNA-dependent synthesis of small de novo products.
January 2010, Biological chemistry,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
De novo initiation of RNA synthesis by the RNA-dependent RNA polymerase (NS5B) of hepatitis C virus.
January 2000, Journal of virology,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
Cyclophilin B stimulates RNA synthesis by the HCV RNA dependent RNA polymerase.
April 2009, Biochemical pharmacology,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
Regulation of de novo-initiated RNA synthesis in hepatitis C virus RNA-dependent RNA polymerase by intermolecular interactions.
June 2010, Journal of virology,
X L Sun, and R B Johnson, and M A Hockman, and Q M Wang
Template nucleotide moieties required for de novo initiation of RNA synthesis by a recombinant viral RNA-dependent RNA polymerase.
November 2000, Journal of virology,
Copied contents to your clipboard!