The infection risk of biomaterials implants varies between different materials and is determined by an interplay of adhesion and surface growth of the infecting organisms. In this study, we compared initial adhesion and surface growth of Staphylococcus epidermidis HBH(2) 102 and Pseudomonas aeruginosa AK1 on poly(dimethylsiloxane), Teflon, polyethylene, polypropylene, polyurethane, poly(ethylene terephthalate), poly(methyl methacrylate), and glass. Initial adhesion was measured in situ in a parallel plate flow chamber with microorganisms suspended in phosphate-buffered saline, while subsequent surface growth was followed in full and in 20 times diluted growth medium. Initial adhesion of both bacterial strains was similar to all biomaterials. In full growth medium, generation times of surface growing S. epidermidis ranged from 17 to 38 min with no relation to wettability, while in diluted growth medium generation times increased from 44 to 98 min with increasing surface wettability. For P. aeruginosa no influence of surface wettability on generation times was observed, but generation times increased with decreasing desorption rates, maximal generation times being 47 min and minimal values down to 30 min. Generally, generation times of adhering bacteria were shorter than of planktonic bacteria. In conclusion, surface growth of initially adhering bacteria is influenced by biomaterials surface properties to a greater extent than initial adhesion.