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In vivo messenger RNA for bacteriophage phiX174 was fractionated in agarose gels into a number of discrete species ranging in size from 0.23 X 10(6) to 2.3 X 10(6) daltons. These RNA species were eluted from the gels and hybridized to specific fragments derived from phiX-174 replicative form DNA by cleavage with restriction enzymes. A map of the orientation of in vivo messenger RNAs with respect to the bacteriophage genetic map was constructed. This map indicated that initiation of messenger RNA occurred before gene B, gene C or D, and probably before gene A and that termination occurred after genes E, F, G, and H. Termination of transcription at any particular site appeared not to be entirely effective such that a number of overlapping transcripts with the same 5'-terminus was observed. Messenger RNA for gene A seemed to be relatively unstable.
M Hayashi, and
F K Fujimura, and
M Hayashi
January 1977,
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M Hayashi, and
F K Fujimura, and
M Hayashi
March 1976,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
M Hayashi, and
F K Fujimura, and
M Hayashi
November 1977,
Nature,
M Hayashi, and
F K Fujimura, and
M Hayashi
July 1980,
The Journal of general virology,
M Hayashi, and
F K Fujimura, and
M Hayashi
November 1966,
Science (New York, N.Y.),
M Hayashi, and
F K Fujimura, and
M Hayashi
July 1967,
Nature,
M Hayashi, and
F K Fujimura, and
M Hayashi
January 1973,
Nature: New biology,
M Hayashi, and
F K Fujimura, and
M Hayashi
June 1973,
Mutation research,
M Hayashi, and
F K Fujimura, and
M Hayashi
July 1972,
Proceedings of the National Academy of Sciences of the United States of America,
M Hayashi, and
F K Fujimura, and
M Hayashi
January 1973,
Nature: New biology,
