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OBJECTIVE The L-type calcium current (ICa,L) in isolated human atrial myocytes was investigated as a possible target of insulin in the regulation of cardiac function. METHODS Atrial myocytes were obtained from patients undergoing cardiac surgery. Using the whole-cell configuration of the patch-clamp technique, we investigated the stimulation of ICa,L by insulin in single human atrial myocytes. RESULTS We found a dose-dependent stimulation of ICa,L by insulin at concentrations of 100 nM, 1 microM and 10 microM. Maximum stimulation of ICa,L over basal ICa,L was 140 +/- 12% (n = 11) at 10 microM insulin. The maximum conductance of ICa,L was increased by 10 microM insulin from 4.0 +/- 0.3 nS to 8.3 +/- 1.0 nS (n = 6). The stimulation of ICa,L by insulin was dose-dependent and reversible. Isoproterenol (10 nM) that stimulates ICa,L by 271 +/- 48% (n = 10) over basal ICa,L acted faster than insulin. The half-maximum stimulation of ICa,L by isoproterenol and insulin (10 microM) was reached after 31 +/- 2 s and 52 +/- 5 s, respectively. The insulin effect shown was totally reversed by acetylcholine (3 microM) which is known to inhibit adenylyl cyclase activity/cAMP-production via Gi-proteins. Also, the selective insulin receptor tyrosine kinase inhibitor (hydroxy-2-naphthanelyl-methyl)phosphonic acid completely inhibited the insulin induced effect. CONCLUSIONS Our data show that insulin stimulates the L-type calcium current in isolated human atrial myocytes in a dose-dependent and reversible manner which appears to involve the insulin receptor tyrosine kinase. Insulin regulation of ICa,L in human atrial myocytes may be an interesting system for the analysis of the metabolic syndrome in man.
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
January 2004,
Receptors & channels,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
December 2022,
Pflugers Archiv : European journal of physiology,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
January 1998,
The Journal of general physiology,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
January 1997,
Journal of cardiovascular pharmacology,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
August 2001,
American journal of physiology. Heart and circulatory physiology,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
April 1996,
Circulation research,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
July 1998,
The American journal of physiology,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
April 2006,
Zhonghua xin xue guan bing za zhi,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
May 2004,
American journal of physiology. Heart and circulatory physiology,
S Maier, and
F Aulbach, and
A Simm, and
V Lange, and
H Langenfeld, and
H Behre, and
U Kersting, and
U Walter, and
M Kirstein
December 2000,
European journal of pharmacology,
