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Temporins, antimicrobial peptides of 10-13 residues, were isolated from secretions of Rana temporaria [Simmaco, M., Mignogna, G., Canofeni, S., Miele, R., Mangoni, M.L. & Barra, D. (1996) Eur. J. Biochem. 242, 788-792]. These molecules are specific to this amphibian species, which is also able to secrete on its skin other antimicrobial peptides similar to those found in different Rana species. The effect of temporins A, B and D (13 residues, net charge +2), and H (10 residues, net charge +1 and +2, respectively) against both artificial membranes of differing lipid composition and bacteria has been investigated in order to gain insight into their mechanisms of action. The results indicate that: the lytic activity of temporins is not greatly affected by the membrane composition; temporins A and B allow the leakage of large-size molecules from the bacterial cells; temporin H renders both the outer and inner membrane of bacteria permeable to hydrophobic substances of low molecular mass; and temporin D, although devoid of antibacterial activity, has a cytotoxic effect on erythrocytes. The results allow important conclusions to be drawn about the minimal structural requirements for lytic efficiency and specificity of temporins.
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
January 2005,
The Journal of biological chemistry,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
October 2008,
Biochemistry,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
November 2000,
Peptides,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
March 2023,
International journal of molecular sciences,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
January 1998,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
January 1994,
Ciba Foundation symposium,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
December 2002,
Current opinion in chemical biology,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
October 2022,
Toxins,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
August 2009,
Biochimica et biophysica acta,
M L Mangoni, and
A C Rinaldi, and
A Di Giulio, and
G Mignogna, and
A Bozzi, and
D Barra, and
M Simmaco
July 2015,
Biochimica et biophysica acta,
