Chronic ethanol ingestion increases efficiency of oxidative phosphorylation in rat liver mitochondria. 2000

M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
Laboratoire de Bioénergétique Fondamentale et Appliquée, UJF, P.O. Box 53X, 38041, Grenoble, France.

The efficiency of oxidative phosphorylation was compared between rats chronically fed with ethanol and controls. (i) Results showed that the liver mitochondria state 4 respiratory rate was strongly inhibited, while the corresponding proton-motive force was not affected; (ii) the cytochrome oxidase content and activity were decreased and (iii) the oxidative-phosphorylation yield was increased in the ethanol exposed group. Furthermore, oxidative phosphorylation at coupling site II was not affected by ethanol. Cytochrome oxidase inhibition by sodium-azide mimicked the effects of ethanol intoxication in control mitochondria. This indicates that the decrease in cytochrome oxidase activity induced by ethanol intoxication directly increases the efficiency of oxidative phosphorylation.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008297 Male Males
D008930 Mitochondria, Liver Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4) Liver Mitochondria,Liver Mitochondrion,Mitochondrion, Liver
D009840 Oligomycins A closely related group of toxic substances elaborated by various strains of Streptomyces. They are 26-membered macrolides with lactone moieties and double bonds and inhibit various ATPases, causing uncoupling of phosphorylation from mitochondrial respiration. Used as tools in cytochemistry. Some specific oligomycins are RUTAMYCIN, peliomycin, and botrycidin (formerly venturicidin X). Oligomycin
D010085 Oxidative Phosphorylation Electron transfer through the cytochrome system liberating free energy which is transformed into high-energy phosphate bonds. Phosphorylation, Oxidative,Oxidative Phosphorylations,Phosphorylations, Oxidative
D010101 Oxygen Consumption The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346) Consumption, Oxygen,Consumptions, Oxygen,Oxygen Consumptions
D002258 Carbonyl Cyanide m-Chlorophenyl Hydrazone A proton ionophore. It is commonly used as an uncoupling agent and inhibitor of photosynthesis because of its effects on mitochondrial and chloroplast membranes. CCCP,Carbonyl Cyanide meta-Chlorophenyl Hydrazone,Carbonylcyanide 4-Chlorophenylhydrazone,Propanedinitrile, ((3-chlorophenyl)hydrazono)-,Carbonyl Cyanide m Chlorophenyl Hydrazone,4-Chlorophenylhydrazone, Carbonylcyanide,Carbonyl Cyanide meta Chlorophenyl Hydrazone,Carbonylcyanide 4 Chlorophenylhydrazone
D003576 Electron Transport Complex IV A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane. Cytochrome Oxidase,Cytochrome aa3,Cytochrome-c Oxidase,Cytochrome Oxidase Subunit III,Cytochrome a,a3,Cytochrome c Oxidase Subunit VIa,Cytochrome-c Oxidase (Complex IV),Cytochrome-c Oxidase Subunit III,Cytochrome-c Oxidase Subunit IV,Ferrocytochrome c Oxygen Oxidoreductase,Heme aa3 Cytochrome Oxidase,Pre-CTOX p25,Signal Peptide p25-Subunit IV Cytochrome Oxidase,Subunit III, Cytochrome Oxidase,p25 Presequence Peptide-Cytochrome Oxidase,Cytochrome c Oxidase,Cytochrome c Oxidase Subunit III,Cytochrome c Oxidase Subunit IV,Oxidase, Cytochrome,Oxidase, Cytochrome-c,Signal Peptide p25 Subunit IV Cytochrome Oxidase,p25 Presequence Peptide Cytochrome Oxidase
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000428 Alcohol Drinking Behaviors associated with the ingesting of ALCOHOLIC BEVERAGES, including social drinking. Alcohol Consumption,Alcohol Intake,Drinking, Alcohol,Alcohol Drinking Habits,Alcohol Drinking Habit,Alcohol Intakes,Consumption, Alcohol,Drinking Habit, Alcohol,Habit, Alcohol Drinking,Habits, Alcohol Drinking,Intake, Alcohol

Related Publications

M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
July 2000, The Biochemical journal,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
July 1964, Archives of biochemistry and biophysics,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
January 1960, Biochimica et biophysica acta,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
February 1957, Biochimica et biophysica acta,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
July 1970, Infection and immunity,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
September 1961, Biochimica et biophysica acta,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
August 1979, The Journal of biological chemistry,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
January 1969, Fiziologia normala si patologica,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
January 1970, Acta vitaminologica et enzymologica,
M A Piquet, and V Nogueira, and A Devin, and B Sibille, and C Filippi, and E Fontaine, and M Roulet, and M Rigoulet, and X M Leverve
March 1970, Archives of biochemistry and biophysics,
Copied contents to your clipboard!