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We have previously described a simple and reproducible method for the measurement of nicotinamide and its major metabolite N-methyl-2-pyridone-5-carboxamide (2-pyr) in human plasma. We now describe a low-cost high-throughput method for measurement of urinary 2-pyr, and demonstrate that Isolute C18 bulk can replace use of the column to clean up the samples prior to injection into the HPLC apparatus. Using a standard curve together with an internal standard for each sample, with mean recovery of 2-pyr greater than 95%, the assay has proved reproducible, with considerable savings in cost and time. The principal advantages of this method are the rapid column clean up of samples prior to injection and the simple but effective methodology.
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
January 1988,
Journal of chromatography,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
June 1987,
Journal of chromatography,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
November 1954,
The Journal of biological chemistry,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
November 1982,
Journal of chromatography,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
January 2014,
Bioscience, biotechnology, and biochemistry,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
July 1963,
Clinica chimica acta; international journal of clinical chemistry,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
January 1958,
Internationale Zeitschrift fur Vitaminforschung. Beiheft,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
May 2003,
Kidney international. Supplement,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
October 2002,
Journal of pharmaceutical and biomedical analysis,
W P Moore, and
C H Bolton, and
L Downs, and
H A Gilmor, and
E A Gale
August 2021,
Journal of chromatographic science,
