-transparent.png){kind=logo}
The transport of palmitic acid (PA) across planar lipid bilayer membranes was measured using a high specific activity [14C]palmitate as tracer for PA. An all-glass trans chamber was employed in order to minimize adsorbance of PA onto the surface. Electrically neutral (diphytanoyl phosphatidylcholine) and charged (Azolectin) planar bilayers were maintained at open electric circuit. We found a permeability to PA of (8.8 +/- 1.9) x 10(-6) cm s(-1) (n = 15) in neutral and of (10.3 +/- 2.2) x 10(-6) cm s(-1) (n = 5) in charged bilayers. These values fall within the order of magnitude of those calculated from desorption constants of PA in different vesicular systems. Differences between data obtained from planar and vesicular systems are discussed in terms of the role of solvent, radius of curvature, and pH changes.
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
February 2003,
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
January 1974,
Methods in enzymology,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
May 2005,
Colloids and surfaces. B, Biointerfaces,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
September 2008,
Proceedings of the National Academy of Sciences of the United States of America,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
February 1975,
Biochimica et biophysica acta,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
September 1970,
Biochimica et biophysica acta,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
December 2008,
Proceedings of the National Academy of Sciences of the United States of America,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
January 1995,
Bioelectromagnetics,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
February 1977,
The Journal of general physiology,
L G Romano-Fontes, and
R Curi, and
C M Peres, and
A Nishiyama-Naruke, and
K Brunaldi, and
F Abdulkader, and
J Procopio
June 2004,
Analytical and bioanalytical chemistry,
