BIOMAT Database Logo BIOMAT Database Logo

Molecular characterization of a novel yeast cell-wall acid phosphatase cloned from Kluyveromyces marxianus. 2000

K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Department of Biotechnology, University of Tokyo, Japan.

A novel Kluyveromyces marxianus gene that encodes an acid phosphatase, Pho610, was cloned in Saccharomyces cerevisiae. The deduced amino acid sequence was distinct from S. cerevisiae phosphatases but similar to some fungal enzymes. A peculiar feature of the sequence is that it has hydrophobic stretches both at the N- and C-termini, which is a characteristic of the precursors of glycosylphosphatidylinositol(GPI)-anchored proteins. When the gene was expressed in S. cerevisiae, the active enzyme was recovered in the periplasmic fraction by glucanase digestion. The Pho610 polypeptide was highly glycosylated and a significant portion was covalently linked to the cell-wall glucan. The enzyme was secreted when the C-terminal region was truncated to remove the GPI signal. Therefore, Pho610 is a novel cell-wall protein having an enzyme activity.

UI MeSH Term Description Entries
D007716 Kluyveromyces An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES. Kluyveromyce
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002473 Cell Wall The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents. Cell Walls,Wall, Cell,Walls, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004693 Glucan Endo-1,3-beta-D-Glucosidase An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman. Endo-1,3-beta-Glucanase,Oligo-1,3-Glucosidase,Zymolase,Endo 1,3 beta Glucanase,Endo-1,3-beta-D-Glucosidase, Glucan,Glucan Endo 1,3 beta D Glucosidase,Oligo 1,3 Glucosidase
D006031 Glycosylation The synthetic chemistry reaction or enzymatic reaction of adding carbohydrate or glycosyl groups. GLYCOSYLTRANSFERASES carry out the enzymatic glycosylation reactions. The spontaneous, non-enzymatic attachment of reducing sugars to free amino groups in proteins, lipids, or nucleic acids is called GLYCATION (see MAILLARD REACTION). Protein Glycosylation,Glycosylation, Protein
D006596 Hexosaminidases Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES. Galactosaminidases,Hexosaminidase,Galactosaminidase,Glucosaminidase,Glucosaminidases
D000135 Acid Phosphatase An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2. Acid beta-Glycerophosphatase,Acid beta Glycerophosphatase
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Induction and characterization of a novel amine oxidase from the yeast Kluyveromyces marxianus.
April 2003, Yeast (Chichester, England),
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Extraction, isolation, structural characterization and prebiotic activity of cell wall polysaccharide from Kluyveromyces marxianus.
August 2022, Carbohydrate polymers,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus.
February 2017, Carbohydrate polymers,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Extraction, purification and characterization of ADH1 from the budding yeast Kluyveromyces marxianus.
January 1990, The Italian journal of biochemistry,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Cell Wall Surface Properties of Kluyveromyces marxianus Strains From Dairy-Products.
January 2019, Frontiers in microbiology,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Advanced structural characterization and in vitro fermentation prebiotic properties of cell wall polysaccharide from Kluyveromyces marxianus.
June 2023, International journal of biological macromolecules,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Purification and partial characterization of Cu/Zn superoxide dismutase from Kluyveromyces marxianus yeast.
November 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Molecular cloning and characterization of an NADPH quinone oxidoreductase from Kluyveromyces marxianus.
September 2003, Journal of biochemistry and molecular biology,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Behaviour of the yeast Kluyveromyces marxianus var. marxianus during its autolysis.
April 1996, Antonie van Leeuwenhoek,
K Yoda, and J H Ko, and T Nagamatsu, and Y Lin, and C Kaibara, and T Kawada, and N Tomishige, and H Hashimoto, and Y Noda, and M Yamasaki
Morphological characterization of the dimorphic yeast Kluyveromyces marxianus var. marxianus NRRLy2415 by semi-automated image analysis.
September 1996, Biotechnology and bioengineering,
Copied contents to your clipboard!