BIOMAT Database Logo BIOMAT Database Logo

Hydrophobic protein that copurifies with human brain acetylcholinesterase: amino acid sequence, genomic organization, and chromosomal localization. 2000

D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Department of Pharmacology, University of Oxford, England. navaratn@mail.med.upenn.edu

The mechanism of attachment of acetylcholinesterase (AChE) to neuronal membranes in interneuronal synapses is poorly understood. We have isolated, sequenced, and cloned a hydrophobic protein that copurifies with AChE from human caudate nucleus and that we propose forms a part of a complex of membrane proteins attached to this enzyme. It is a short protein of 136 amino acids and has a molecular mass of 18 kDa. The sequence contains stretches of both hydrophobic and hydrophilic amino acids and two cysteine residues. Analysis of the genomic sequence reveals that the coding region is divided among five short exons. Fluorescence in situ hybridization localizes the gene to chromosome 6p21.32-p21.2. Northern blot analysis shows that this gene is widely expressed in the brain with an expression pattern that parallels that of AChE.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000110 Acetylcholinesterase An enzyme that catalyzes the hydrolysis of ACETYLCHOLINE to CHOLINE and acetate. In the CNS, this enzyme plays a role in the function of peripheral neuromuscular junctions. EC 3.1.1.7. Acetylcholine Hydrolase,Acetylthiocholinesterase,Hydrolase, Acetylcholine
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D015152 Blotting, Northern Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Northern Blotting,Blot, Northern,Northern Blot,Blots, Northern,Blottings, Northern,Northern Blots,Northern Blottings
D016678 Genome The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA. Genomes

Related Publications

D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Genomic sequence, organization, and chromosomal localization of human JAK3.
October 1996, Genomics,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Genomic organization, promoter, amino acid sequence, chromosomal localization, and expression of the human gene for CORS-26 (collagenous repeat-containing sequence of 26-kDa protein).
November 2003, Biochimica et biophysica acta,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Human myosin-binding protein H (MyBP-H): complete primary sequence, genomic organization, and chromosomal localization.
April 1993, Genomics,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Genomic organization and chromosomal localization of the human SGK protein kinase gene.
July 1998, Genomics,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Genomic organization, sequence, and chromosomal localization of the human helix-loop-helix Id1 gene.
February 1997, Biochemical and biophysical research communications,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
The human lysozyme gene. Sequence organization and chromosomal localization.
July 1989, European journal of biochemistry,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Human gamma-aminobutyraldehyde dehydrogenase (ALDH9): cDNA sequence, genomic organization, polymorphism, chromosomal localization, and tissue expression.
June 1996, Genomics,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2-interacting transmembrane adaptor protein (SIT).
January 2001, Immunogenetics,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Cloning, genomic organization, and chromosomal localization of human cathepsin L.
January 1993, The Journal of biological chemistry,
D S Navaratnam, and F S Fernando, and J D Priddle, and K Giles, and S M Clegg, and D J Pappin, and I Craig, and A D Smith
Genomic organization and chromosomal localization of the human nucleolin gene.
September 1990, The Journal of biological chemistry,
Copied contents to your clipboard!