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A method for measuring brush border membrane enzymes from small intestinal biopsies by crossed immunoelectrophoresis is presented. The use of a brush border specific antiserum made isolation of the brush border membrane before analysis unnecessary. This prevented loss of material which, together with inactivation of enzymes, was a limiting factor in previous studies of brush border enzymes from peroral biopsies. In 58 biopsies from patients without gastrointestinal disorders a close correlation between antigenic activity and corresponding enzymatic activity was shown for the following enzymes: sucrase-isomaltase (EC 3.2.1.48-EC 3.2.1.10), lactase-phlorizin hydrolase (EC 3.2.1.23-EC 3.2.1.62), microvillus aminopeptidase (microsomal, EC 3.4.11.2) and dipeptidyl peptidase IV (EC 3.4.14.X). The immunoelectrophoretic patterns of intestinal mucosa near the ligament of Treitz, and in jejunum and ileum were established. The method presented is thought to be of value in further studies of the molecular basis of brush border diseases.
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
March 1981,
The Biochemical journal,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
October 1977,
Biochimica et biophysica acta,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
May 1981,
Clinica chimica acta; international journal of clinical chemistry,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
March 1981,
Pediatric research,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
May 1980,
Gut,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
January 1999,
Acta gastro-enterologica Belgica,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
January 1981,
Biochimie,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
June 1978,
Clinica chimica acta; international journal of clinical chemistry,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
January 1998,
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition,
H Skovbjerg, and
H Sjöström, and
O Norén, and
E Gudmand-Høyer
November 1997,
Molecular biology of the cell,
