The habituation of auditory P300 event-related brain potential (ERP) from single-stimulus paradigm was evaluated and compared to that from oddball paradigm. Three task conditions were: oddball with a button press (oddball/press) response, single-stimulus with a button-press (single-stimulus/press) response and a silent count (single-stimulus/count) response. The oddball/press condition demonstrated larger P300 amplitude and longer latency overall than either single-stimulus condition, but P300 amplitude decreased and peak latency increased similarly over successive trial blocks for all three tasks. Thus, the oddball and single-stimulus ERP tasks produce analogous changes under repeated measurements and indicate that the single-stimulus task can serve as an alternative method for eliciting the P300 in applied and clinical settings.