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The binding site of puromycin was probed chemically in the peptidyl-transferase center of ribosomes from Escherichia coli and of puromycin-hypersensitive ribosomes from the archaeon Haloferax gibbonsii. Several nucleotides of the 23S rRNAs showed altered chemical reactivities in the presence of puromycin. They include A2439, G2505, and G2553 for E. coli, and G2058, A2503, G2505, and G2553 for Hf. gibbonsii (using the E. coli numbering system). Reproducible enhanced reactivities were also observed at A508 and A1579 within domains I and III, respectively, of E. coli 23S rRNA. In further experiments, puromycin was shown to produce a major reduction in the UV-induced crosslinking of deacylated-(2N3A76)tRNA to U2506 within the P' site of E. coli ribosomes. Moreover, it strongly stimulated the putative UV-induced crosslink between a streptogramin B drug and m2A2503/psi2504 at an adjacent site in E. coli 23S rRNA. These data strongly support the concept that puromycin, along with other peptidyl-transferase antibiotics, in particular the streptogramin B drugs, bind to an RNA structural motif that contains several conserved and accessible base moieties of the peptidyl transferase loop region. This streptogramin motif is also likely to provide binding sites for the 3' termini of the acceptor and donor tRNAs. In contrast, the effects at A508 and A1579, which are located at the exit site of the peptide channel, are likely to be caused by a structural effect transmitted along the peptide channel.
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
November 1975,
Proceedings of the National Academy of Sciences of the United States of America,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
July 1977,
European journal of biochemistry,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
January 1993,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
April 2006,
Antimicrobial agents and chemotherapy,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
February 1997,
Journal of molecular biology,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
October 2021,
Biophysical chemistry,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
November 2010,
Antimicrobial agents and chemotherapy,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
June 2009,
Journal of bacteriology,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
June 1979,
FEBS letters,
C Rodriguez-Fonseca, and
H Phan, and
K S Long, and
B T Porse, and
S V Kirillov, and
R Amils, and
R A Garrett
November 1973,
Biochemical and biophysical research communications,
