Granulocyte-macrophage colony-stimulating factor upregulates matrix metalloproteinase-2 (MMP-2) and membrane type-1 MMP (MT1-MMP) in human head and neck cancer cells. 2000

T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
Department of Otolaryngology, Keio University, School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160-8582, Tokyo, Japan.

Matrix metalloproteinase-2 (MMP-2) and membrane type 1-MMP (MT1-MMP) play an important role in the invasion and metastasis of head and neck squamous cell carcinoma (HNSCC), but the mechanism of their regulation is not clearly understood. Recently, granulocyte-macrophage colony-stimulating factor (GM-CSF) has been shown to be associated with cancer invasion and metastasis. We hypothesized that GM-CSF may upregulate MMP-2 and/or MT1-MMP expression in HNSCC cells, and may thereby influence their ability to invade and metastasize. We studied the effects of GM-CSF on the production of MMP-2 and MT1-MMP in HNSCC cell lines SAS and HSC-2. Gelatin zymography of conditioned media derived from HNSCC cells revealed a major band of 68 kDa, which was characterized as proMMP-2. GM-CSF stimulated the production of proMMP-2 in both cell lines in a dose-dependent manner. Treatment with 50 ng/ml GM-CSF for 24 h increased the proMMP-2 activity 3.4-fold in SAS cells and 2.3-fold in HSC-2 cells compared with untreated controls. Northern blot analyses demonstrated that GM-CSF led to elevated mRNA levels of MMP-2 and MT1-MMP in both cell lines. The results identify GM-CSF as a regulator of MMP-2 and MT1-MMP expression in certain types of HNSCC, and suggest that GM-CSF may contribute to the invasiveness of HNSCC through the regulation of MMP-2 and MT1-MMP expression.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008666 Metalloendopeptidases ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism. Metallo-Endoproteinases,Metalloendopeptidase
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D006258 Head and Neck Neoplasms Soft tissue tumors or cancer arising from the mucosal surfaces of the LIP; oral cavity; PHARYNX; LARYNX; and cervical esophagus. Other sites included are the NOSE and PARANASAL SINUSES; SALIVARY GLANDS; THYROID GLAND and PARATHYROID GLANDS; and MELANOMA and non-melanoma skin cancers of the head and neck. (from Holland et al., Cancer Medicine, 4th ed, p1651) Cancer of Head and Neck,Head Cancer,Head Neoplasm,Head and Neck Cancer,Head and Neck Neoplasm,Neck Cancer,Neck Neoplasm,Neck Neoplasms,Neoplasms, Upper Aerodigestive Tract,UADT Neoplasm,Upper Aerodigestive Tract Neoplasm,Upper Aerodigestive Tract Neoplasms,Cancer of Head,Cancer of Neck,Cancer of the Head,Cancer of the Head and Neck,Cancer of the Neck,Head Neoplasms,Head, Neck Neoplasms,Neoplasms, Head,Neoplasms, Head and Neck,Neoplasms, Neck,UADT Neoplasms,Cancer, Head,Cancer, Neck,Cancers, Head,Cancers, Neck,Head Cancers,Neck Cancers,Neoplasm, Head,Neoplasm, Neck,Neoplasm, UADT,Neoplasms, UADT
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D016178 Granulocyte-Macrophage Colony-Stimulating Factor An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. CSF-GM,Colony-Stimulating Factor, Granulocyte-Macrophage,GM-CSF,Histamine-Producing Cell-Stimulating Factor,CSF-2,TC-GM-CSF,Tumor-Cell Human GM Colony-Stimulating Factor,Cell-Stimulating Factor, Histamine-Producing,Colony Stimulating Factor, Granulocyte Macrophage,Granulocyte Macrophage Colony Stimulating Factor,Histamine Producing Cell Stimulating Factor,Tumor Cell Human GM Colony Stimulating Factor
D016187 Receptors, Granulocyte-Macrophage Colony-Stimulating Factor Receptors that bind and internalize the granulocyte-macrophage stimulating factor. Their MW is believed to be 84 kD. The most mature myelomonocytic cells, specifically human neutrophils, macrophages, and eosinophils, express the highest number of affinity receptors for this growth factor. Antigens, CD116,CD116 Antigens,GM-CSF Receptors,CD116 Antigen,GM-CSF Receptor,Receptor, Granulocyte-Macrophage Colony-Stimulating Factor,Receptors, GM-CSF,Antigen, CD116,GM CSF Receptor,GM CSF Receptors,Receptor, GM-CSF,Receptor, Granulocyte Macrophage Colony Stimulating Factor,Receptors, GM CSF,Receptors, Granulocyte Macrophage Colony Stimulating Factor
D053504 Matrix Metalloproteinases, Membrane-Associated Matrix metalloproteinases that are associated with the CELL MEMBRANE, either through transmembrane domains or GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORS. Membrane-type matrix metalloproteinases may act within the pericellular environment to influence the process of CELL MIGRATION. Membrane-Type Matrix Metalloproteinase,MT-MMP Enzymes,Matrix Metalloproteinase, Membrane-Type,Membrane-Type Matrix Metalloproteinases,MT MMP Enzymes,Matrix Metalloproteinase, Membrane Type,Matrix Metalloproteinases, Membrane Associated,Matrix Metalloproteinases, Membrane-Type,Membrane Type Matrix Metalloproteinase,Membrane Type Matrix Metalloproteinases,Membrane-Associated Matrix Metalloproteinases,Metalloproteinase, Membrane-Type Matrix,Metalloproteinases, Membrane-Associated Matrix,Metalloproteinases, Membrane-Type Matrix

Related Publications

T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
January 2011, International journal of clinical and experimental medicine,
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
June 2000, Journal of Korean medical science,
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
July 1992, The New England journal of medicine,
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
July 1992, The New England journal of medicine,
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
March 2008, International journal of cancer,
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
March 2023, Archives of oral biology,
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
June 2000, Journal of immunology (Baltimore, Md. : 1950),
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
August 2000, Current eye research,
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
July 1987, Journal of immunology (Baltimore, Md. : 1950),
T Tomita, and M Fujii, and Y Tokumaru, and Y Imanishi, and M Kanke, and T Yamashita, and R Ishiguro, and J Kanzaki, and K Kameyama, and Y Otani
July 2004, Journal of cellular physiology,
Copied contents to your clipboard!