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In vivo receptor labeling of the peripheral benzodiazepine receptor was investigated using ex vivo binding of [3H]-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinoline-carb ox-amide ([3H]PK11195). In autoradiographic studies, high level specific binding of [3H]PK11195 was observed in the olfactory bulb. Intravenous administration of PK11195 dose-dependently (0.03-3 mg/kg) inhibited ex vivo binding of [3H]PK11195 in the olfactory bulb. Likewise, N-(2,5-dimethoxybenzyl)-N-(5-fluoro-2-phenoxyphenyl)acetamide (DAA1106), a newly identified peripheral benzodiazepine receptor-specific ligand, dose-dependently (0.1-100 mg/kg) reduced ex vivo binding of [3H]PK11195, when administered intraperitoneally. In contrast, clonazepam, a central benzodiazepine receptor-specific agonist, had negligible effects on ex vivo binding of [3H]PK11195. We propose that the ex vivo receptor binding technique we used will facilitate determination of in vivo receptor occupancy of the peripheral benzodiazepine receptor.
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
September 1985,
Kaku igaku. The Japanese journal of nuclear medicine,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
May 1994,
Digestive diseases and sciences,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
July 1990,
Journal of neurochemistry,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
July 1985,
Life sciences,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
September 1994,
Metabolic brain disease,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
July 1995,
European journal of pharmacology,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
January 1994,
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
July 2000,
Naunyn-Schmiedeberg's archives of pharmacology,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
August 1992,
The Journal of pharmacy and pharmacology,
T Funakoshi, and
S Chaki, and
S Okuyama, and
T Okubo, and
A Nakazato, and
M Nagamine, and
K Tomisawa
February 1997,
Journal of neurocytology,
