Ran alters nuclear pore complex conformation. 2000

M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslow Road, Manchester, M20 9BX, UK. mgoldberg@picr.man.ac.uk

Transport across the nuclear membranes occurs through the nuclear pore complex (NPC), and is mediated by soluble transport factors including Ran, a small GTPase that is generally GDP-bound during import and GTP-bound for export. The dynamic nature of the NPC structure suggests a possible active role for it in driving translocation. Here we show that RanGTP but not RanGDP causes alterations of NPC structure when injected into the cytoplasm of Xenopus oocytes, including compaction of the NPC and extension of the cytoplasmic filaments. RanGTP caused accumulation of nucleoplasmin-gold along the length of extended cytoplasmic filaments, whereas RanGDP caused accumulation around the cytoplasmic rim of the NPC. This suggests a possible role for Ran in altering the conformation of the cytoplasmic filaments during transport.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D009685 Nuclear Envelope The membrane system of the CELL NUCLEUS that surrounds the nucleoplasm. It consists of two concentric membranes separated by the perinuclear space. The structures of the envelope where it opens to the cytoplasm are called the nuclear pores (NUCLEAR PORE). Nuclear Membrane,Envelope, Nuclear,Envelopes, Nuclear,Membrane, Nuclear,Membranes, Nuclear,Nuclear Envelopes,Nuclear Membranes
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D009994 Osmolar Concentration The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent. Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D010750 Phosphoproteins Phosphoprotein
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D011993 Recombinant Fusion Proteins Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. Fusion Proteins, Recombinant,Recombinant Chimeric Protein,Recombinant Fusion Protein,Recombinant Hybrid Protein,Chimeric Proteins, Recombinant,Hybrid Proteins, Recombinant,Recombinant Chimeric Proteins,Recombinant Hybrid Proteins,Chimeric Protein, Recombinant,Fusion Protein, Recombinant,Hybrid Protein, Recombinant,Protein, Recombinant Chimeric,Protein, Recombinant Fusion,Protein, Recombinant Hybrid,Proteins, Recombinant Chimeric,Proteins, Recombinant Fusion,Proteins, Recombinant Hybrid
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms

Related Publications

M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
October 2025, Cell,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
December 2004, Biophysical chemistry,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
February 2005, Eukaryotic cell,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
February 2020, Journal of cell science,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
July 2008, Structure (London, England : 1993),
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
November 1997, The Journal of cell biology,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
March 2003, The Journal of cell biology,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
November 2002, European journal of cell biology,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
May 1995, Trends in cell biology,
M W Goldberg, and S A Rutherford, and M Hughes, and L A Cotter, and S Bagley, and E Kiseleva, and T D Allen, and P R Clarke
September 2002, Biophysical journal,
Copied contents to your clipboard!