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RNA ligase isolated from Escherichia coli infected with bacteriophage T4 will catalyze the formation of an intermolecular 3' leads to 5' phosphodiester linkage between an oligoribonucleotide with a free 3'-hydroxyl and another oligoribonucleotide with a 5'-phosphate. Upon reaction with (Ap)5C, nearly quantitative conversion of the hexamer [5'-32P]p(Up)5U to the dodecamer (Ap)5C[3' leads to 5'-32P]p(Up)5U was observed. The product was identified by its mobility on RPC-5 column chromatography, its resistance to alkaline phosphatase, and the appearance of the expected radiolabeled products on hydrolysis with alkali, ribonuclease A, snake venom phosphodiesterase, and spleen phosphodiesterase. The coupling of other pairs of single-stranded oligoribonucleotides has also been demonstrated. The intermolecular joining reaction is probably mechanistically similar to the intramolecular cyclization activity previously reported for Tr RNA ligase. It is expected that this enzyme will be useful for the synthesis of RNA fragments of defined sequence.
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
January 1977,
Nucleic acids research,
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
October 1986,
Analytical biochemistry,
G C Walker, and
O C Uhlenbeck, and
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R I Gumport
February 1980,
Biochemistry,
G C Walker, and
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E Bedows, and
R I Gumport
January 1995,
Biochimie,
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
November 1978,
Biochemistry,
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
June 1991,
Bioorganicheskaia khimiia,
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
January 1985,
Analytical biochemistry,
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
September 1997,
Nucleic acids research,
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
January 1998,
Molecular diversity,
G C Walker, and
O C Uhlenbeck, and
E Bedows, and
R I Gumport
December 2005,
The FEBS journal,
