Biomaterial Database

Characterisation of reaction centers and their phospholipids from Rhodospirillum rubrum. 1979

M Snozzi, and R Bachofen

1. Reaction centers from Rhodospirillum rubrum have been extracted with the zwitterionic detergent lauryl dimethyl amine oxide. Subsequent purification has been achieved by gel filtration and ion-exchange chromatography. The pure reaction centers are composed of three protein subunits (L, M, H), bacteriocholorophyll and bacteriopheophytin in the ratio 2 : 1 and phospholipids. 2. The phospholipid composition has been found to be similar to that of whole chromatophore membrane, except that diphosphatidyl glycerol is present in higher amount in the isolated complex. When the detergent treatment of the chromatophore membrane is done in the presence of NaCl, a lower phospholipid content in isolated reaction centers has been found together with a lower stability in the association among the protein subunits. In this complex, the largest subunit H is easily split off and a LM complex is obtained. It is concluded that the phospholipids play an important role in the stability of reaction center complexes.

UI	MeSH Term	Description	Entries
D007425	Intracellular Membranes	Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.	Membranes, Intracellular,Intracellular Membrane,Membrane, Intracellular
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D010860	Pigments, Biological	Any normal or abnormal coloring matter in PLANTS; ANIMALS or micro-organisms.	Biological Pigments
D002458	Cell Fractionation	Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.	Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D003902	Detergents	Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.	Cleansing Agents,Detergent Pods,Laundry Detergent Pods,Laundry Pods,Syndet,Synthetic Detergent,Agent, Cleansing,Agents, Cleansing,Cleansing Agent,Detergent,Detergent Pod,Detergent Pod, Laundry,Detergent Pods, Laundry,Detergent, Synthetic,Detergents, Synthetic,Laundry Detergent Pod,Laundry Pod,Pod, Detergent,Pod, Laundry,Pod, Laundry Detergent,Pods, Detergent,Pods, Laundry,Pods, Laundry Detergent,Synthetic Detergents
D004123	Dimethylamines	Derivatives of dimethylamine (the structural formula CH3NHCH3).
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D012247	Rhodospirillum rubrum	Vibrio- to spiral-shaped phototrophic bacteria found in stagnant water and mud exposed to light.