Decomposition of DDT and its analogs by soil microflora. 1978

G K Skryabin, and L A Golovleva, and E L Golovlev, and R N Pertsova, and A M Zyakun, and Y V Shurukhin, and B P Strekozov

A search was made for active cultures that break down DDT. More than 600 microorganisms were isolated from soils treated for a long time with DDT and from accumulating cultures with DDT or its analogs as the carbon source and with DDT or its analogs and supplementary carbon sources. Cultures capable of degrading DDT under conditions of cometabolism were found among them. It was shown that the dechlorination of the trichloromethyl group of DDT was accomplished by a large number of microorganisms and occurred primarily under conditions of limited aeration. The elimination of chloride ions from the aromatic rings occurred only in the presence of cosubstrates under aerobic conditions and only with two strains. The strain Nocardia sp. 306x carried out complete degradation of DDT with the formation of DBH, DCBP, and an as yet unidentified entirely dechlorinated product. The most profound decomposition of DDT was produced by a culture of Ps. aeruginosa 640x, which entirely degraded the DDT molecule with the formation of phenylacetic acid.

UI MeSH Term Description Entries
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D003634 DDT A polychlorinated pesticide that is resistant to destruction by light and oxidation. Its unusual stability has resulted in difficulties in residue removal from water, soil, and foodstuffs. This substance may reasonably be anticipated to be a carcinogen: Fourth Annual Report on Carcinogens (NTP-85-002, 1985). (From Merck Index, 11th ed) 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane,4,4'-DDT,4,4'-Dichlorodiphenyltrichloroethane,Benzochloryl,Chlorophenothane,TbisC-ethane,p',p'-DDT,4,4' DDT,4,4' Dichlorodiphenyltrichloroethane,TbisC ethane
D001673 Biodegradation, Environmental Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers. Bioremediation,Phytoremediation,Natural Attenuation, Pollution,Environmental Biodegradation,Pollution Natural Attenuation
D012988 Soil Microbiology The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms. Microbiology, Soil

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