-transparent.png){kind=logo}
The interaction of brilliant cresol blue (BCB) with nucleic acids in aqueous solution has been studied by spectrophotometry and Rayleigh light scattering (RLS) spectroscopy. Under suitable conditions, the RLS spectra of BCB changed significantly due to the presence of nucleic acids. RLS intensity of BCB at 364 nm is greatly enhanced with the addition of nucleic acids, and a new RLS peak is observed at 552 nm. This peak is about half the intensity of that at 364 nm. The results of this study show that BCB interacts with DNA possibly due to the cooperative effect of electrostatic attraction, intercalation, coordination and hydrophobic effect. Under optimum conditions, the increase of RLS at 364 nm of a BCB solution is proportional to the concentration of nucleic acids added. This result is the basis for a new RLS method for determination of nucleic acids. The linear range of ctDNA, fsDNA and yRNA is 0.12-4.70, 0.11-4.64 and 0.43-7.07 microg ml(-1), respectively.
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
April 1999,
Guang pu xue yu guang pu fen xi = Guang pu,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
January 2002,
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
February 1987,
Photochemistry and photobiology,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
January 2006,
Annali di chimica,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
November 1976,
Zeitschrift fur die gesamte innere Medizin und ihre Grenzgebiete,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
February 1989,
Photochemistry and photobiology,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
May 2001,
Current protocols in protein science,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
July 1999,
Talanta,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
October 1959,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
Y T Wang, and
F L Zhao, and
K A Li, and
S Y Tong
January 1948,
The Biochemical journal,
