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Treponema pallidum were extracted from testicular syphilomas of corticosteroid-treated rabbits and purified by differential centrifugation. The steroid therapy allowed a longer holding time for infected rabbits, which produced greater treponeme yields, averaging 1.58 x 10(10) treponemes per rabbit. The treatment, which also diminished cellular infiltration and increased the extracellular mucoid material in lesions, produced much cleaner suspensions than preparations from nontreated animals. Most of the treponemes in the purified suspensions were still motile, and none carried demonstrable host immunoglobulin. The preparations were free of recognizable host tissue debris and they contained, on the average, 1.9 x 10(-7) mug of protein per treponeme.
P H Hardy, and
E E Nell
January 1987,
Acta dermato-venereologica,
P H Hardy, and
E E Nell
August 1993,
Acta dermato-venereologica,
P H Hardy, and
E E Nell
September 1936,
American journal of public health and the nation's health,
P H Hardy, and
E E Nell
February 1986,
Genitourinary medicine,
P H Hardy, and
E E Nell
August 1953,
Public health reports (Washington, D.C. : 1896),
P H Hardy, and
E E Nell
September 1984,
Infection and immunity,
P H Hardy, and
E E Nell
August 1971,
The British journal of venereal diseases,
P H Hardy, and
E E Nell
January 1972,
Archiv fur dermatologische Forschung,
P H Hardy, and
E E Nell
April 1971,
The British journal of venereal diseases,
P H Hardy, and
E E Nell
March 1967,
Experientia,
