Biomaterial Database

Construction and phenotypes of double sporulation deficient mutants in Streptomyces coelicolor A3(2). 1975

K F Chater

In whiA, B, G and H mutants of Streptomyces coelicolor A3(2), aerial hyphae develop but sporulation speta are not formed. Septa are produced by whiI mutants but are spaced abnormally far apart. Mutants in each locus have a distinctive aerial mycelium morphology, except for whiA and B mutants which are closely similar. Seven strains were made with pairwise combinations of whiA and B mutations with whiG, H and I mutations and with each other. The genotypes of these strains were confirmed by suitable crosses and their aerial mycelium morphology examined. An indirect procedure was used to determine the aerial mycelium morphology of whiGH, GI and HI double mutants. The double mutants always closely resembled one of the single mutant parent strains in morphology and a consistent scheme of epistasis was obtained--whiG being epistatic to whiH, A, B and I; whiH to whiA, B and I; and whiA or B to whiI. These results point to the absence of any complex interactions between gene products, which might have been revealed by the occurrence of novel phenotypes in double mutants or by inconsistencies in the epistasis scheme.

UI	MeSH Term	Description	Entries
D008040	Genetic Linkage	The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.	Genetic Linkage Analysis,Linkage, Genetic,Analyses, Genetic Linkage,Analysis, Genetic Linkage,Genetic Linkage Analyses,Linkage Analyses, Genetic,Linkage Analysis, Genetic
D008858	Microscopy, Phase-Contrast	A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.	Phase-Contrast Microscopy,Microscopies, Phase-Contrast,Microscopy, Phase Contrast,Phase Contrast Microscopy,Phase-Contrast Microscopies
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D011995	Recombination, Genetic	Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.	Genetic Recombination,Recombination,Genetic Recombinations,Recombinations,Recombinations, Genetic
D002874	Chromosome Mapping	Any method used for determining the location of and relative distances between genes on a chromosome.	Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D003433	Crosses, Genetic	Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.	Cross, Genetic,Genetic Cross,Genetic Crosses
D013172	Spores, Fungal	Reproductive bodies produced by fungi.	Conidia,Fungal Spores,Conidium,Fungal Spore,Spore, Fungal
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.