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We have established a simplified assay system for the measurement of urinary kallikrein activity by utilizing the sensitive and specific radioimmunoassay system of kinins previously reported from our laboratory. Kinins were generated by incubating urine samples (50 microliter) with kininogen (1500 ng) in the presence of kininase inhibitors, and the generated kinins were measured by radioimmunoassay. Since the cross reactivity of kininogen in the kinin radioimmunoassay system was not recognized at dose up to 1.0 microgram, the amount of untreated kininogen in the radioimmunoassay samples did not interfere with the measurement of kinins. This eliminated the necessity for a kininogen extraction procedure. A good linear correlation (r = 0.939, p less than 0.001) was observed between the urinary kallikrein activity determined by this assay system (kininogenase activity) and that by esterolytic acitvity. Urinary kallikrein activity was 3.3 +/- 0.9 microgram/min/24 hour urine (mean +/- SEM), 1.4 +/- 0.4 microgram/min/24 hour urine and 0.25 +/- 0.06 microgram/min/24 hour urine in 6 normal subjects, 7 patients with non-complicated essential hypertension and 4 patients with chronic renal failure, respectively. Thus, urinary kallikrein activity was significantly lower in the patients with essential hypertension (p less than 0.05) and the patients with chronic renal failure (p less than 0.01) than in the normal subjects.
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S Tanaka, and
T Nakao, and
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Y Nakahashi, and
M Sakuma, and
M Miyahara
October 1976,
Biochemical pharmacology,
K Shimamoto, and
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M Sakuma, and
M Miyahara
November 1981,
Biochimica et biophysica acta,
K Shimamoto, and
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M Miyahara
July 1980,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
K Shimamoto, and
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T Nakao, and
T Ando, and
Y Nakahashi, and
M Sakuma, and
M Miyahara
January 1978,
Klinische Wochenschrift,
K Shimamoto, and
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Y Nakahashi, and
M Sakuma, and
M Miyahara
June 2000,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
K Shimamoto, and
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M Sakuma, and
M Miyahara
January 1979,
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K Shimamoto, and
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M Miyahara
September 1987,
The Journal of sports medicine and physical fitness,
K Shimamoto, and
S Tanaka, and
T Nakao, and
T Ando, and
Y Nakahashi, and
M Sakuma, and
M Miyahara
June 1977,
Journal of steroid biochemistry,
K Shimamoto, and
S Tanaka, and
T Nakao, and
T Ando, and
Y Nakahashi, and
M Sakuma, and
M Miyahara
August 1973,
Journal of clinical pathology,
K Shimamoto, and
S Tanaka, and
T Nakao, and
T Ando, and
Y Nakahashi, and
M Sakuma, and
M Miyahara
August 1971,
Biochemical pharmacology,
