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We have found the two-dimensional electrophoretic technique of O'Farrell to be highly efficient in the detection of charge-change substitutions in a large number of proteins. We have applied this method to determine the level of heterozygosity of the most abundant proteins in Drosophila melanogaster adults from a natural population. The estimate of per-locus heterozygosity obtained from approximately 54 loci screened was 4% with 6 loci polymorphic. This is much lower than overall estimates obtained by standard gel electrophoresis but is not different from estimates for "Group I" enzymes--i.e., those utilizing a narrow spectrum of substrates of intracellular origin. We consider these data to throw open the question of the level of genetic variability in nature.
A J Brown, and
C H Langley
May 1992,
Molecular biology and evolution,
A J Brown, and
C H Langley
February 1980,
Nature,
A J Brown, and
C H Langley
August 1991,
Biochemical genetics,
A J Brown, and
C H Langley
January 1978,
Journal of molecular biology,
A J Brown, and
C H Langley
March 1969,
Proceedings of the National Academy of Sciences of the United States of America,
A J Brown, and
C H Langley
July 1991,
Nucleic acids research,
A J Brown, and
C H Langley
October 1987,
Genetics,
A J Brown, and
C H Langley
December 1983,
Biochemical genetics,
A J Brown, and
C H Langley
December 1967,
Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie,
A J Brown, and
C H Langley
August 1973,
Journal of mammalogy,
