-transparent.png){kind=logo}
Using 4-month-old fetal bovine tissue, the properties of the tibia epiphyseal cartilage matrix vesicles, a type of endochondral ossification tissue, were compared with those from tracheal cartilage. The matrix vesicle fractions, obtained by collagenase digestion and differential centrifugation, were subjected to sucrose-density-gradient centrifugation. Alkaline phosphatase activity, protease activity, and lacatate dehydrogenase activity were assayed for the marker enzyme of the matrix vesicles. Matrix vesicles containing alkaline phosphatase, metalloprotease, and lacatate dehydrogenase were found in the tibia epiphyseal cartilage at a density of 1.11 g/ml. In surprising contrast, we also found matrix vesicle-like vesicles with a high density of 1.24 g/ml in the tracheal cartilage. These also contained alkaline phosphatase and lactate dehydrogenase, but not metalloprotease. The electrophoretic profiles of the lactate dehydrogenase isoenzymes from the matrix vesicle and matrix vesicle-like vesicles were identical with those of chondrocyte cytosolic lactate dehydrogenase. Aldolase, aspartate: 2-oxoglutarate aminotransferase, alanine: 2-oxoglutarate aminotransferase, glucose-6-phosphatase, glutamate dehydrogenase, catalase, and cytosolic enzymes except for lactate dehydrogenase were not detected in these vesicles. These results suggest the presence of a mechanism for specific uptake of cytosolic lactate dehydrogenase in both vesicles. In this study, a new type of matrix vesicles without protease was found in the tracheal cartilage, a kind of permanent cartilage, but not in the tibia epiphyseal cartilage, which is replaced by bone tissue.
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
August 1978,
Proceedings of the National Academy of Sciences of the United States of America,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
May 1974,
Calcified tissue research,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
February 1976,
Federation proceedings,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
January 1979,
Journal of electron microscopy,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
January 1986,
The International journal of biochemistry,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
June 2007,
Acta physiologica (Oxford, England),
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
September 1984,
Calcified tissue international,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
February 1976,
Federation proceedings,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
January 1978,
Acta anatomica,
K Maki, and
S Hayashi, and
T Nishioka, and
M Kimura, and
T Noguch
September 1993,
Histochemistry,
