BIOMAT Database Logo BIOMAT Database Logo

Repression of virus-induced interferon A promoters by homeodomain transcription factor Ptx1. 2000

S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Laboratoire de Régulation de la Transcription et Maladies Génétiques, CNRS, UPR 2228, UFR Biomédicale des Saints-Pères, Université René Descartes, 75270 Paris Cedex 06, France. Sebastien.Navarro@biomedicale.univ-paris5.fr

Interferon A (IFN-A) genes are differentially expressed after virus induction. The differential expression of individual IFN-A genes is modulated by substitutions in the proximal positive virus responsive element A (VRE-A) of their promoters and by the presence or absence of a distal negative regulatory element (DNRE). The functional feature of the DNRE is to specifically act by repression of VRE-A activity. With the use of the yeast one-hybrid system, we describe here the identification of a specific DNRE-binding protein, the pituitary homeobox 1 (Ptx1 or Pitx1). Ptx1 is detectable in different cell types that differentially express IFN-A genes, and the endogenous Ptx1 protein binds specifically to the DNRE. Upon virus induction, Ptx1 negatively regulates the transcription of DNRE-containing IFN-A promoters, and the C-terminal region, as well as the homeodomain of the Ptx1 protein, is required for this repression. After virus induction, the expression of the Ptx1 antisense RNA leads to a significant increase of endogenous IFN-A gene transcription and is able to modify the pattern of differential expression of individual IFN-A genes. These studies suggest that Ptx1 contributes to the differential transcriptional strength of the promoters of different IFN-A genes and that these genes may provide new targets for transcriptional regulation by a homeodomain transcription factor.

UI MeSH Term Description Entries
D007370 Interferon Type I Interferon secreted by leukocytes, fibroblasts, or lymphoblasts in response to viruses or interferon inducers other than mitogens, antigens, or allo-antigens. They include alpha- and beta-interferons (INTERFERON-ALPHA and INTERFERON-BETA). Interferons Type I,Type I Interferon,Type I Interferons,Interferon, Type I,Interferons, Type I
D009522 Newcastle disease virus The most well known avian paramyxovirus in the genus AVULAVIRUS and the cause of a highly infectious pneumoencephalitis in fowl. It is also reported to cause CONJUNCTIVITIS in humans. Transmission is by droplet inhalation or ingestion of contaminated water or food. Avian Paramyxovirus 1,Paramyxovirus 1, Avian
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D012097 Repressor Proteins Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release. Repressor Molecules,Transcriptional Silencing Factors,Proteins, Repressor,Silencing Factors, Transcriptional
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Modulation of interferon expression by hepatitis C virus NS5A protein and human homeodomain protein PTX1.
February 2003, Virology,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Repression by homeoprotein pitx1 of virus-induced interferon a promoters is mediated by physical interaction and trans repression of IRF3 and IRF7.
October 2002, Molecular and cellular biology,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Repression of regulatory factor for Drosophila DNA replication-related gene promoters by zerknüllt homeodomain protein.
January 1994, The Journal of biological chemistry,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Active repression of transcription by the engrailed homeodomain protein.
June 1991, The EMBO journal,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6.
January 1998, Molecular and cellular biology,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor.
December 2018, Molecular cell,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
The POU transcription factor Oct-1 represses virus-induced interferon A gene expression.
October 2005, Molecular and cellular biology,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Control of cotton fibre elongation by a homeodomain transcription factor GhHOX3.
November 2014, Nature communications,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene.
May 1996, Genes & development,
S Lopez, and M L Island, and J Drouin, and M T Bandu, and N Christeff, and N Darracq, and R Barbey, and J Doly, and D Thomas, and S Navarro
Downregulation of vaccinia virus intermediate and late promoters by host transcription factor YY1.
July 2009, The Journal of general virology,
Copied contents to your clipboard!