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OBJECTIVE Rat chromosome 20 is one of special interest because it contains some diabetogenic genes, such as a major histocompatibilitiy complex (MHC)-linked genetic components and quantitative trait loci. We studied rat chromosome 20, using the backcross progeny between BB/Wor and PVG.R23 rats, and confirmed the genetic linkage map by use of another backcross panel. METHODS Backcross panels were done between BB/Wor and PVG.R23 rats, and BN and KZC rats. Length variations of simple sequence length polymorphism markers were analyzed by use of polymerase chain reaction (PCR) analysis. Alleles of RT1-Bb and RT1-Db were analyzed by use of the PCR-restriction fragment length polymorphism method. Genetic maps of rat chromosome 20 were constructed, using the Map Manager computer program. RESULTS Fifty-two loci were mapped on rat chromosome 20. Genetic length was 57.9 cM, with average spanning of 1.11 cM between markers. The positions of RT1-N1, Tnf, and RT1-Bb into the MHC region were separated and confirmed by results of two backcross panels in our linkage studies. CONCLUSIONS The genetic linkage map of rat chromosome 20 was improved, and was a useful tool for genetic analysis of a diabetogenic gene(s) and for producing MHC congenic strains.
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
October 1998,
Immunogenetics,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
November 1989,
Genomics,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
July 1998,
Mammalian genome : official journal of the International Mammalian Genome Society,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
February 1992,
Genomics,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
January 1985,
Annals of the New York Academy of Sciences,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
April 1994,
Mammalian genome : official journal of the International Mammalian Genome Society,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
January 1993,
Genomics,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
August 1988,
Genomics,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
September 1990,
Genomics,
T Masuyama, and
J Ishibiki, and
T Awata, and
M Noda, and
Y Kanazawa, and
M Sugawara, and
K Komeda
January 1999,
Mammalian genome : official journal of the International Mammalian Genome Society,
