BIOMAT Database Logo BIOMAT Database Logo

Metabolic labeling of glycoproteins with chemical tags through unnatural sialic acid biosynthesis. 2000

C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Department of Chemistry, University of California at Berkeley 94720, USA.

UI MeSH Term Description Entries
D007659 Ketones Organic compounds containing a carbonyl group Ketone
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell

Related Publications

C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Bifunctional unnatural sialic acids for dual metabolic labeling of cell-surface sialylated glycans.
June 2013, Journal of the American Chemical Society,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I.
September 2008, Journal of the American Chemical Society,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Quantum dots with phenylboronic acid tags for specific labeling of sialic acids on living cells.
February 2011, Analytical chemistry,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Cellular metabolism of unnatural sialic acid precursors.
October 2015, Glycoconjugate journal,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
End-labeling oligonucleotides with chemical tags after synthesis.
January 2012, Methods in molecular biology (Clifton, N.J.),
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Metabolic labeling of glycoproteins with radioactive sugars.
February 2002, Current protocols in cell biology,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Sialic Acid Glycoengineering Using an Unnatural Sialic Acid for the Detection of Sialoglycan Biosynthesis Defects and On-Cell Synthesis of Siglec Ligands.
October 2015, ACS chemical biology,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Structure-Activity Relationship of Metabolic Sialic Acid Inhibitors and Labeling Reagents.
March 2022, ACS chemical biology,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Biosynthesis and metabolic degradation of sphingolipids not containing sialic acid.
May 1972, Journal of lipid research,
C L Jacobs, and K J Yarema, and L K Mahal, and D A Nauman, and N W Charters, and C R Bertozzi
Targeted metabolic labeling of yeast N-glycans with unnatural sugars.
March 2010, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!