-transparent.png){kind=logo}
A dielectric method has already been developed for the real-time monitoring of cell cycle progression in synchronized cell culture (Asami et al., 1999). This method, in combination with DNA content analysis by fluorescence-activated cell sorting (FACS), was applied to the synchronized cell culture of a CDC28-13th mutant (Saccharomyces cerevisiae). In synchronous cell growth, relative permittivity epsilon (or dielectric constant) for the culture broth showed cyclic changes at low frequencies below 0.5 MHz, being correlated to phases in the cell cycle that were simultaneously determined by FACS. The epsilon increased in the period from S phase to G(2) phase and decreased between M and G(1) phases. Peaks in these cyclic changes of epsilon indicated the time when daughter cells segregated from mother cells.
K Asami, and
K Takahashi, and
K Shirahige
February 1992,
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine,
K Asami, and
K Takahashi, and
K Shirahige
June 1982,
Cancer research,
K Asami, and
K Takahashi, and
K Shirahige
February 1988,
The American journal of pathology,
K Asami, and
K Takahashi, and
K Shirahige
January 1994,
In vitro cellular & developmental biology. Animal,
K Asami, and
K Takahashi, and
K Shirahige
December 1991,
Gastroenterology,
K Asami, and
K Takahashi, and
K Shirahige
February 2021,
Analytical biochemistry,
K Asami, and
K Takahashi, and
K Shirahige
July 1994,
Cytometry,
K Asami, and
K Takahashi, and
K Shirahige
December 1983,
Mutation research,
K Asami, and
K Takahashi, and
K Shirahige
January 1993,
Anticancer research,
K Asami, and
K Takahashi, and
K Shirahige
March 1991,
Zhonghua zhong liu za zhi [Chinese journal of oncology],
