BIOMAT Database Logo BIOMAT Database Logo

Polymorphism in the gene coding for the immunodominant antigen gp43 from the pathogenic fungus Paracoccidioides brasiliensis. 2000

F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
Departamento de Microbiologia, Imunologia e Parasitologia da Universidade Federal de São Paulo, São Paulo, SP, Brazil.

The gp43 glycoprotein is an immune-dominant antigen in patients with paracoccidioidomycosis (PCM). It is protective against murine PCM and is a putative virulence factor. The gp43 gene of Paracoccidioides brasiliensis B-339 is located in a 1,329-bp DNA fragment that includes two exons, a 78-bp intron, and a leader peptide-coding region of 105 bp. Polymorphism in gp43 has been suggested by the occurrence, in the same isolate or among different fungal samples, of isoforms with distinct isoelectric points. In the present study we aligned and compared with a consensus sequence the gp43 precursor genes of 17 P. brasiliensis isolates after sequencing two PCR products from each fungal sample. The genotypic types detected showed 1 to 4 or 14 to 15 informative substitution sites, preferentially localized between 578 and 1166 bp. Some nucleotide differences within individual isolates (noninformative sites) resulted in a second isoelectric point for the deduced protein. The most polymorphic sequences were also phylogenetically distant from the others and encoded basic gp43 isoforms. The three isolates in this group were from patients with chronic PCM, and their DNA restriction patterns were distinct in Southern blots. The nucleotides encoding the inner core of the murine T-cell-protective epitope of gp43 were conserved, offering hope for the development of a universal vaccine.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009844 Oligosaccharides Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form. Oligosaccharide
D010228 Paracoccidioides A mitosporic fungal genus. P. brasiliensis (previously Blastomyces brasiliensis) is the etiologic agent of PARACOCCIDIOIDOMYCOSIS. Blastomyces brasiliensis,Loboa loboi,Paracoccidioides brasiliensis
D010229 Paracoccidioidomycosis A mycosis affecting the skin, mucous membranes, lymph nodes, and internal organs. It is caused most often by Paracoccidioides brasiliensis. It is also called paracoccidioidal granuloma. Blastomyces brasiliensis Infections,Blastomycosis, South American,Paracoccidioides Infection,Paracoccidioides Infections,Paracoccidioidomycoses,Blastomyces brasiliensis Infection,Paracoccidioidal Granuloma,Paracoccidioides brasiliensis Infection,Granuloma, Paracoccidioidal,Infection, Blastomyces brasiliensis,Infection, Paracoccidioides,Infection, Paracoccidioides brasiliensis,Infections, Paracoccidioides brasiliensis,Paracoccidioidal Granulomas,Paracoccidioides brasiliensis Infections,Paracoccidioidomycose,South American Blastomycosis
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
GP43 from Paracoccidioides brasiliensis inhibits macrophage functions. An evasion mechanism of the fungus.
January 2002, Cellular immunology,
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
The kex2 gene from the dimorphic and human pathogenic fungus Paracoccidioides brasiliensis.
October 2002, Yeast (Chichester, England),
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
Paracoccidioides brasiliensis-gp43 used as paracoccidioidin.
January 1996, Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology,
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
DNA-based vaccination against murine paracoccidioidomycosis using the gp43 gene from paracoccidioides brasiliensis.
July 2000, Vaccine,
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
Functional genome of the human pathogenic fungus Paracoccidioides brasiliensis.
September 2005, FEMS immunology and medical microbiology,
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
Towards a molecular genetic system for the pathogenic fungus Paracoccidioides brasiliensis.
December 2007, Fungal genetics and biology : FG & B,
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
Expression of a kexin-like gene from the human pathogenic fungus Paracoccidioides brasiliensis in Saccharomyces cerevisiae.
June 2008, Medical mycology,
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
General metabolism of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis.
June 2005, Genetics and molecular research : GMR,
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
Molecular cloning and characterization of a glucan synthase gene from the human pathogenic fungus Paracoccidioides brasiliensis.
March 2000, Yeast (Chichester, England),
F V Morais, and T F Barros, and M K Fukada, and P S Cisalpino, and R Puccia
Screening non-coding RNAs in transcriptomes from neglected species using PORTRAIT: case study of the pathogenic fungus Paracoccidioides brasiliensis.
August 2009, BMC bioinformatics,
Copied contents to your clipboard!