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NM23.H1 is a protein connected with tumor progression. Loss of heterozygosity and reduced expression of the gene have been associated with poor prognosis and increased incidence of metastases in many epithelial tumors. The aim of this study was to detect the presence of NM23.H1 point mutations or small deletions in human breast carcinomas by using the single-strand-conformation polymorphism (SSCP) technique. Mutational analysis was performed on 76 breast tumors, 10 of which had allelic deletion of the gene. The NM23.H1 mRNA content also was evaluated in each sample. Only a C-to-A transversion leading to a stop codon was found in the 5' untranslated region of exon 1. A polymorphic SSCP pattern was identified in exon 1; direct sequencing showed a C-to-T transition 30 nucleotides upstream from the 5' splice site flanking exon 1. None of the tumors analyzed presented both alleles inactivated. Our results suggest that NM23.H1 is rarely inactivated by point mutations.
G Cipollini, and
A Moretti, and
C Ghimenti, and
P Viacava, and
G Bevilacqua, and
M A Caligo
June 2000,
Zhongguo fei ai za zhi = Chinese journal of lung cancer,
G Cipollini, and
A Moretti, and
C Ghimenti, and
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G Bevilacqua, and
M A Caligo
January 1996,
Oncology reports,
G Cipollini, and
A Moretti, and
C Ghimenti, and
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G Bevilacqua, and
M A Caligo
December 1994,
British journal of cancer,
G Cipollini, and
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M A Caligo
January 1998,
Anticancer research,
G Cipollini, and
A Moretti, and
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G Bevilacqua, and
M A Caligo
January 1998,
Oncology,
G Cipollini, and
A Moretti, and
C Ghimenti, and
P Viacava, and
G Bevilacqua, and
M A Caligo
July 2004,
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences,
G Cipollini, and
A Moretti, and
C Ghimenti, and
P Viacava, and
G Bevilacqua, and
M A Caligo
October 1990,
Pathologie-biologie,
G Cipollini, and
A Moretti, and
C Ghimenti, and
P Viacava, and
G Bevilacqua, and
M A Caligo
July 1999,
Zhonghua yi xue za zhi = Chinese medical journal; Free China ed,
G Cipollini, and
A Moretti, and
C Ghimenti, and
P Viacava, and
G Bevilacqua, and
M A Caligo
February 1997,
Chinese medical journal,
G Cipollini, and
A Moretti, and
C Ghimenti, and
P Viacava, and
G Bevilacqua, and
M A Caligo
January 2007,
In vivo (Athens, Greece),
