BIOMAT Database Logo BIOMAT Database Logo

Automated image analysis for monitoring oxidative burst in macrophages. 2000

B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Department of Radiobiology, National Atomic Energy Commission, Buenos Aires, Argentina. beamolin@cnea.gov.ar

OBJECTIVE To evaluate oxidative bursts induced by phorbol myristate acetate in phagocytes at the single-cell level by automated image analysis. METHODS The generation of reactive oxygen species was quantitatively expressed by means of histograms displaying the percentage of cells corresponding to each of the total optical densities measured. RESULTS Macrophage subpopulations were quantitatively defined. This method allows detailed analysis of the amount of formazan per cell and the sites of deposition of blue precipitate in each cell. CONCLUSIONS Image analysis is a reliable quantitative, single-cell assay for studying various cellular characteristics associated with macrophage functions.

UI MeSH Term Description Entries
D007091 Image Processing, Computer-Assisted A technique of inputting two-dimensional or three-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer. Biomedical Image Processing,Computer-Assisted Image Processing,Digital Image Processing,Image Analysis, Computer-Assisted,Image Reconstruction,Medical Image Processing,Analysis, Computer-Assisted Image,Computer-Assisted Image Analysis,Computer Assisted Image Analysis,Computer Assisted Image Processing,Computer-Assisted Image Analyses,Image Analyses, Computer-Assisted,Image Analysis, Computer Assisted,Image Processing, Biomedical,Image Processing, Computer Assisted,Image Processing, Digital,Image Processing, Medical,Image Processings, Medical,Image Reconstructions,Medical Image Processings,Processing, Biomedical Image,Processing, Digital Image,Processing, Medical Image,Processings, Digital Image,Processings, Medical Image,Reconstruction, Image,Reconstructions, Image
D010586 Phagocytes Cells that can carry out the process of PHAGOCYTOSIS. Phagocyte,Phagocytic Cell,Phagocytic Cells,Cell, Phagocytic,Cells, Phagocytic
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013755 Tetradecanoylphorbol Acetate A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA. Phorbol Myristate Acetate,12-Myristoyl-13-acetylphorbol,12-O-Tetradecanoyl Phorbol 13-Acetate,Tetradecanoylphorbol Acetate, 4a alpha-Isomer,12 Myristoyl 13 acetylphorbol,12 O Tetradecanoyl Phorbol 13 Acetate,13-Acetate, 12-O-Tetradecanoyl Phorbol,Acetate, Phorbol Myristate,Acetate, Tetradecanoylphorbol,Myristate Acetate, Phorbol,Phorbol 13-Acetate, 12-O-Tetradecanoyl,Tetradecanoylphorbol Acetate, 4a alpha Isomer
D016676 Macrophages, Alveolar Round, granular, mononuclear phagocytes found in the alveoli of the lungs. They ingest small inhaled particles resulting in degradation and presentation of the antigen to immunocompetent cells. Alveolar Macrophages,Macrophages, Pulmonary,Pulmonary Macrophages,Macrophage, Pulmonary,Pulmonary Macrophage,Alveolar Macrophage,Macrophage, Alveolar
D016897 Respiratory Burst A large increase in oxygen uptake by neutrophils and most types of tissue macrophages through activation of an NADPH-cytochrome b-dependent oxidase that reduces oxygen to a superoxide. Individuals with an inherited defect in which the oxidase that reduces oxygen to superoxide is decreased or absent (GRANULOMATOUS DISEASE, CHRONIC) often die as a result of recurrent bacterial infections. Oxidative Burst,Burst, Oxidative,Burst, Respiratory,Bursts, Oxidative,Bursts, Respiratory,Oxidative Bursts,Respiratory Bursts
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D017382 Reactive Oxygen Species Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of SIGNAL TRANSDUCTION and GENE EXPRESSION, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS. Active Oxygen Species,Oxygen Radical,Oxygen Radicals,Pro-Oxidant,Reactive Oxygen Intermediates,Active Oxygen,Oxygen Species, Reactive,Pro-Oxidants,Oxygen, Active,Pro Oxidant,Pro Oxidants,Radical, Oxygen

Related Publications

B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
An automated micro-fluorometric assay for monitoring oxidative burst activity of phagocytes.
February 1993, Journal of immunological methods,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Pneumocystis carinii induces an oxidative burst in alveolar macrophages.
January 1992, Infection and immunity,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Oxidative burst and metallothionein as a scavenger in macrophages.
June 2001, Immunology and cell biology,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Capsaicin inhibits calmodulin-mediated oxidative burst in rat macrophages.
January 1990, Cellular signalling,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Platelet activating factor (PAF) induces the oxidative burst in macrophages.
January 1983, International journal of immunopharmacology,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Automated image analysis for bladder cancer.
January 1998, Urological research,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Inference systems for automated image analysis.
November 1992, Dento maxillo facial radiology,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Automated monitoring of activated sludge in a pilot plant using image analysis.
January 2001, Water science and technology : a journal of the International Association on Water Pollution Research,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Neutrophil oxidative burst capacity for peri-operative immune monitoring in trauma patients.
August 2014, Injury,
B L Molinari, and D R Tasat, and M L Fernández, and H A Durán, and J Curiale, and A Stoliar, and R L Cabrini
Automated Diabetic Retinopathy Screening and Monitoring Using Retinal Fundus Image Analysis.
February 2016, Journal of diabetes science and technology,
Copied contents to your clipboard!