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The influence of different concentrations of chloramphenicol on the RNA synthesis was studied in E. coli strains CP 78 (rel+) and CP 79 (rel-). In cells growing in the medium with succinate as a carbon source the maximal stimulation of RNA synthesis was obtained in the presence of 5-10 mug/ml of chloramphenicol-that is when the protein synthesis in the cells is decreased to 50-30%. Concentrations of antibiotic 50-100 mug/ml only slightly stimulate the incorporation of 14C-uracil in TCA-insoluble fraction. The high level of RNA synthesis once achieved in the presence of small doses of chloramphenicol was not affected by higher concentrations of antibiotic up to 105 mug/ml. It is suggested that at low doses of chloramphenicol some proteins of positive control of RNA synthesis are synthesized. Under amino acids starvation in the presence of succinate only rel+ cells stopped RNA synthesis. The phenotypic suppression of RNA synthesis with chloramphenicol in these cells is achieved only by high concentrations of antibiotic (50-100 mug/ml). It is proposed that under amino acid starvation RNA synthesis is independent on the protein formation and is determined by relative contents of programmed ribosomes in cells.
R C Shchakupov, and
E V Kpiachko
February 1973,
Nature: New biology,
R C Shchakupov, and
E V Kpiachko
January 1976,
Acta hepato-gastroenterologica,
R C Shchakupov, and
E V Kpiachko
October 1979,
FEBS letters,
R C Shchakupov, and
E V Kpiachko
October 1973,
Archives internationales de physiologie et de biochimie,
R C Shchakupov, and
E V Kpiachko
October 1970,
FEBS letters,
R C Shchakupov, and
E V Kpiachko
May 1975,
Antimicrobial agents and chemotherapy,
R C Shchakupov, and
E V Kpiachko
January 1974,
Planta,
R C Shchakupov, and
E V Kpiachko
January 1966,
Biochemical and biophysical research communications,
R C Shchakupov, and
E V Kpiachko
January 1974,
The Journal of cell biology,
