BIOMAT Database Logo BIOMAT Database Logo

Extrachromosomal deoxyribonucleic acid in R factor-harboring Enterobacteriaceae. 1976

J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup

Extrachromosomal deoxyribonucleic acid (DNA) from 24 different R factor-harboring Enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. The R factors represented 15 different patterns of transferable drug resistance found in enterobacteria from an enclosed geographic area. All of the strains contained extrachromosomal, circular DNA molecules within the range of 0.4 to 52 mum. More than one size class of circular DNA molecules was observed in the majority of the extrachromosomal DNA preparations. The buoyant density of the extrachromosomal DNA ranged from 1.700 to 1.720 g/cm3. The majority of the bacteria contained extrachromosomal DNAs of various densities. Three-fourths of the R factors were classified as fi+. The investigation illustrates the extensive variability in the physical characteristics of plasmid DNA from R factor-harboring strains.

UI MeSH Term Description Entries
D007711 Klebsiella pneumoniae Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans. Bacillus pneumoniae,Bacterium pneumoniae crouposae,Hyalococcus pneumoniae,Klebsiella pneumoniae aerogenes,Klebsiella rhinoscleromatis
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D011513 Proteus mirabilis A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that is frequently isolated from clinical specimens. Its most common site of infection is the urinary tract.
D011815 R Factors A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation. R Factor,R Plasmid,R Plasmids,Resistance Factor,Resistance Factors,Factor, R,Factor, Resistance,Factors, R,Factors, Resistance,Plasmid, R,Plasmids, R
D003718 Denmark A country in northern Europe, bordering the Baltic Sea and the North Sea. The capital is Copenhagen. Faeroe Islands,Faroe Islands
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004270 DNA, Circular Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992) Circular DNA,Circular DNAs,DNAs, Circular
D004352 Drug Resistance, Microbial The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS). Antibiotic Resistance,Antibiotic Resistance, Microbial,Antimicrobial Resistance, Drug,Antimicrobial Drug Resistance,Antimicrobial Drug Resistances,Antimicrobial Resistances, Drug,Drug Antimicrobial Resistance,Drug Antimicrobial Resistances,Drug Resistances, Microbial,Resistance, Antibiotic,Resistance, Drug Antimicrobial,Resistances, Drug Antimicrobial
D004755 Enterobacteriaceae A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock. Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D004756 Enterobacteriaceae Infections Infections with bacteria of the family ENTEROBACTERIACEAE. Enterobacterial Infections,Cronobacter Infections,Infections, Enterobacteriaceae,Infections, Enterobacterial,Cronobacter Infection,Enterobacteriaceae Infection,Enterobacterial Infection,Infection, Cronobacter,Infection, Enterobacteriaceae,Infection, Enterobacterial,Infections, Cronobacter

Related Publications

J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
Extrachromosomal deoxyribonucleic acid in different enterobacteria.
April 1973, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
[EPISOMIC RESISTANCE FACTOR IN ENTEROBACTERIACEAE. 31. THE DEGRADATION OF BACTERIOPHAGE DEOXYRIBONUCLEIC ACID BY FI-R FACTORS].
July 1965, Igaku to seibutsugaku. Medicine and biology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
Denaturation mapping of R factor deoxyribonucleic acid.
September 1975, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
Deoxyribonucleic acid-deoxyribonucleic acid hybridization of R factors.
September 1973, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
Composite circular forms of R factor deoxyribonucleic acid molecules.
January 1969, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
R factor deoxyribonucleic acid in chromosomeless progeny of Escherichia coli.
February 1971, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
R factor-controlled restriction and modification of deoxyribonucleic acid: restriction mutants.
December 1972, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells.
July 1970, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
Specific labeling and physical characterization of R-factor deoxyribonucleic acid in Escherichia coli.
October 1970, Journal of bacteriology,
J K Moller, and A L Bak, and C Christiansen, and G Christiansen, and A Stenderup
Molecular nature of R-factor deoxyribonucleic acid isolated from Salmonella typhimurium minicells.
June 1973, Journal of bacteriology,
Copied contents to your clipboard!