BIOMAT Database Logo BIOMAT Database Logo

Posttransplant infusion of donor-specific blood induces immunological unresponsiveness in rat hepatic allografts. 2000

J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Department of Surgery II, Kumamoto University Medical School, Japan.

BACKGROUND We previously reported that pretransplant donor-specific blood transfusion (DST) induces CD45RC-CD4+ T cells, Th2-like effector cells, and prolongs rat hepatic allograft survival. Our study investigated the effects of posttransplant DST on rat hepatic allograft survival. METHODS Three days after transplantation, LEW (RT1(1)) recipient rats with ACI (RT1a) livers were injected i.v. with freshly heparinized donor-specific blood. The time kinetics of CD45RC-CD4+ and CD45RC+CD4+ T cell subsets in hepatic infiltrates were examined. RESULTS Posttransplant DST significantly prolonged rat hepatic allograft survival. Interferon (IFN)-gamma, interleukin (IL)-12, and IL-18 mRNA levels in hepatic allografts of untreated recipients were significantly greater than in recipients treated with posttransplant DST. However, hepatic allografts of recipients treated with posttransplant DST showed significantly higher IL-4, IL-10, and transforming growth factor (TGF)-beta mRNA levels than untreated recipients. The ratio of CD45RC-CD4+ T cells to CD45RC+CD4+ T cells was significantly higher in hepatic allografts treated with posttransplant DST than in untreated animals. Immunostaining with anti-rat dendritic cell (OX-62) monoclonal antibody revealed that OX-62+ cells were distributed to the splenic red pulp of animals treated with posttransplant DST and to the splenic white pulp in untreated animals. Most OX62+ cells isolated from the spleen of recipients treated with posttransplant DST expressed donor RT1Ba class II major histocompatibility complex antigens, suggesting that OX-62+ cells were of donor origin. CONCLUSIONS Posttransplant DST was associated with persistent infiltration of CD45RC-CD4+ T cells, Th2-like effector cells, in rat hepatic allografts, causing immunologic unresponsiveness and establishment of microchimerism in the spleen.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297 Male Males
D011912 Rats, Inbred ACI An inbred strain of rat that is widely used in BIOMEDICAL RESEARCH. Applications include the study of spontaneous NEOPLASMS; CHRONIC KIDNEY DISEASES, and CONGENITAL ABNORMALITIES. Rats, Inbred A x C 9935 Irish,Rats, ACI,ACI Rat,ACI Rat, Inbred,ACI Rats,ACI Rats, Inbred,Inbred ACI Rat,Inbred ACI Rats,Rat, ACI,Rat, Inbred ACI
D011917 Rats, Inbred Lew An inbred strain of rat that is used in BIOMEDICAL RESEARCH. Rats, Inbred Lewis,Rats, Lew,Inbred Lew Rat,Inbred Lew Rats,Inbred Lewis Rats,Lew Rat,Lew Rat, Inbred,Lew Rats,Lew Rats, Inbred,Lewis Rats, Inbred,Rat, Inbred Lew,Rat, Lew
D001803 Blood Transfusion The introduction of whole blood or blood component directly into the blood stream. (Dorland, 27th ed) Blood Transfusions,Transfusion, Blood,Transfusions, Blood
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D004396 Coloring Agents Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS. Coloring Agent,Dye,Dyes,Organic Pigment,Stain,Stains,Tissue Stain,Tissue Stains,Organic Pigments,Pigments, Inorganic,Agent, Coloring,Inorganic Pigments,Pigment, Organic,Pigments, Organic,Stain, Tissue,Stains, Tissue
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006085 Graft Survival The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host. Graft Survivals,Survival, Graft,Survivals, Graft
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Immunological unresponsiveness to hepatic allografts in rats. Immunological reactivities of the recipient to donor antigens.
June 1989, Transplantation,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Failure of nonimmunogenic islet allografts to induce donor-specific immunological unresponsiveness [corrected].
January 1994, Cell transplantation,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Induction of donor-specific unresponsiveness in rat islet allografts by FK 506.
June 1992, Transplantation proceedings,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Awareness of donor alloantigens in antiadhesion therapy induces antigen-specific unresponsiveness to islet allografts.
October 1997, Transplantation,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
CD45RC gammadelta T-cell infiltration is associated with immunologic unresponsiveness induced by prior donor-specific blood transfusion in rat hepatic allografts.
April 2001, Hepatology (Baltimore, Md.),
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Differential splenic migration of dendritic cells after immunologic unresponsiveness in rat hepatic allografts induced by pretransplant donor-specific transfusion.
November 2001, The Journal of surgical research,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Induction of donor specific unresponsiveness in rat islet allografts by intraportal grafting and FK506.
January 1994, Cell transplantation,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Bone marrow cell- and FK 506-induced donor-specific unresponsiveness in rat heart allografts.
December 1991, Transplantation proceedings,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Injection of mitomycin-C-treated spleen cells induces donor-specific unresponsiveness to cardiac allografts in rats.
March 1999, Transplantation,
J Liang, and Y Yamaguchi, and T Matsuda, and H Ohshiro, and J L Zhang, and K Okabe, and F Matsumura, and K Ishihara, and S Uchino, and K Mori, and S Yamada, and M Ogawa
Induction of specific unresponsiveness to rat islet allografts by intrathymic UVB donor spleen cells.
November 1993, Transplantation,
Copied contents to your clipboard!