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A new senitive and specific micromethod for determination of UDP-glucuronic acid is described Extracts from 2.5 mg of liver are incubated with twice washed guinea pig microsomes (as a source of UDP-glucuronyl transferase) and [14C]p-nitrophenol. The content of UDP-glucuronic acid is calculated from the [14C]p-nitrophenyl glucuronide/[14C]p-nitrophenol radioactivity ratio and the known amount of introduced [14C]p-nitrophenol. These values are corrected for losses of UDP-glucuronic acid by a calibration experiment, containing in addition to the aforementioned constituents, a known amount of UDP-glucuronic acid. The mean concentration of UDP-glucuronic acid in mice liver was found to be 171 mumol/kg wet weight.
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
November 1979,
Die Pharmazie,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
July 1975,
Biochemical pharmacology,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
January 1981,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
August 1979,
Clinica chimica acta; international journal of clinical chemistry,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
December 1977,
Zeitschrift fur Lebensmittel-Untersuchung und -Forschung,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
December 1977,
Rinsho byori. The Japanese journal of clinical pathology,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
November 1978,
Nihon yakurigaku zasshi. Folia pharmacologica Japonica,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
August 1980,
The Biochemical journal,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
January 1978,
Arzneimittel-Forschung,
W Lehnert, and
T O Wagner, and
M W Fürst, and
W Künzer
November 1967,
Biochimica et biophysica acta,
