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The Syrian hamster embryo (SHE) cell transformation assay evaluates the potential of chemicals to induce morphological transformation in karyotypically normal primary cells. Induction of transformation has been shown to correlate well with the carcinogenicity of many compounds in the rodent bioassay. Historically the assay has not received wide-spread use due to technical difficulty. An improved protocol for a low pH 6.7 assay was developed by LeBoeuf et al. [R.A. LeBoeuf, G.A. Kerckaert, M.J. Aardema, D.P. Gibson, R. Brauninger, R.J. Isfort, Mutat. Res., 356 (1996) 85-127], that greatly reduced many of the technical difficulties associated with the SHE assay. The purpose of this paper is to describe the most current execution of the pH 6.70 protocol including protocol refinements made since the publication of a comprehensive protocol for this assay in Kerckaert et al. [G.A. Kerckaert, R.J. Isfort, G.J. Carr, M.J. Aardema, Mutat. Res., 356 (1996) 65-84].
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
February 2008,
Current protocols in toxicology,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
April 2012,
Mutation research,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
September 1996,
Mutation research,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
April 2012,
Mutation research,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
September 1996,
Mutation research,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
April 2012,
Mutation research,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
September 1996,
Mutation research,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
August 2013,
Mutation research,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
May 2012,
Mutagenesis,
L Custer, and
D P Gibson, and
M J Aardema, and
R A LeBoeuf
May 2016,
Mutation research. Genetic toxicology and environmental mutagenesis,
