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[Typing of isolated mycobacteria]. 2000

S Zvizdić, and I Masić
Institut za mikrobiologiju, Medicinski fakultet Univerziteta u Sarajevu.

Microbiological diagnosis of causes of pulmonic or other tuberculosis localization includes microscopic analysis of disease material cause which was taken earlier, his specific elaboration and inoculation at appropriate bases. Bases are incubated until 12 weeks. In the purpose of identification of M. tuberculosis complex or some other (conditionally) potentially pathogenous king of micobacterium, depending on immunity status of the investigated one, it is necessary to apply conventional as well as modern methods of molecular biology in laboratory work. First of all this means differentiation of M. tuberculosis complex from other bacterium (MOT), that is eventually differentiation inside complex. Beside classical tests it is possible to apply some methods of molecular biology in laboratory conditions, as well as nucleus sonds or method for reproduction of one part of nucleus acid which fragments, with a help of restrictive encimes, into several parts (PCR/PRA). With this method it is possible to do complete identification of isolated kinds of micobacterium according to the size of aplicon's fragments (part of reproduces DNA). An appropriate time, which is necessary for execution of these tests, is measured in hours and days, and for the classical tests in weeks and months.

UI MeSH Term Description Entries
D009161 Mycobacterium A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts. Mycobacteria
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015336 Molecular Probe Techniques The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms. Molecular Probe Technic,Molecular Probe Technics,Molecular Probe Technique,Technic, Molecular Probe,Technics, Molecular Probe,Technique, Molecular Probe,Techniques, Molecular Probe,Probe Technic, Molecular,Probe Technics, Molecular,Probe Technique, Molecular,Probe Techniques, Molecular
D015373 Bacterial Typing Techniques Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping. Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

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